Functional Analysis of the Menkes Protein (MNK) Expressed from a cDNA Construct
A detailed structure/function analysis of the Menkes protein (MNK) is required to elucidate its role in maintaining cellular copper homeostasis. It was recently demonstrated that over-expression of the MNK protein confers a copper-resistance phenotype upon CHO-K1 cells (Camakaris et al, 1995), and that MNK in these cells is located primarily in the trans-Golgi network (Petris et al., 1996), but trafflcks to the plasma membrane in elevated copper levels. To investigate the molecular mechanism of the copper-induced trafficking of MNK, and copper translocation across cellular membranes, stable expression of the full length protein in mammalian cells is necessary, followed by the generation of cell lines that contain in vitro mutated MNK constructs.
KeywordsWilson Disease Cystic Fibrosis Transmembrane Regulatory Copper Transport cDNA Construct Menkes Disease
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