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Uncoupling of Respiration and Phosphorylation

  • Chapter
Frontiers of Cellular Bioenergetics

Abstract

Initially, the term uncoupling was introduced to define the respiration processes proceeding without phosphorylation. Such a phenomenon was discovered by Belitser and Tsibakova in 1939. The authors found that the addition of arsenate to a skeletal muscle mince allowed respiration to occur without ATP formation (Belitser and Tsibakova, 1939). However, later, when the role of ΔμH+ and ΔμNa+ as convertible energy currencies was elucidated, the definition of the uncoupling phenomenon was changed. Now, three types of cellular respiration are distinguished:

  1. 1.

    Energy-coupled respiration. This is respiration generating Δμ̄H+ or Δμ̄Na+, which are then utilized to perform useful work, namely, chemical work (e.g., ATP synthesis), osmotic work (uphill transport of solutes), or mechanical work (rotation of bacterial flagellum).

  2. 2.

    Uncoupled respiration. As in (1), but Δμ̄H+ or Δμ̄Na+ formed are immediately dissipated with no work performed.

  3. 3.

    Noncoupled respiration. In this case respiration occurs in such a way that neither Δμ̄H+ or Δμ̄Na+ are formed.

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  1. Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119899, Russia

    Vladimir P. Skulachev

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  1. Vladimir P. Skulachev
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  1. University of Bari, Bari, Italy

    Sergio Papa  & Ferruccio Guerrieri  & 

  2. University of Amsterdam, Amsterdam, The Netherlands

    Joseph M. Tager

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Skulachev, V.P. (1999). Uncoupling of Respiration and Phosphorylation. In: Papa, S., Guerrieri, F., Tager, J.M. (eds) Frontiers of Cellular Bioenergetics. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4843-0_4

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