Abstract
Cellular drug resistance is thought to be an important cause of the poor prognosis in childhood leukemia.4 Drug resistance in leukemic cells may occur primary or may be acquired. To develop more effective treatment regimens for resistance patients is necessary, but it is difficult to use many drugs at same time in patients.2,3 Therefore, it is more easy to use in vitro systems for various drug treatment of cells so that individual chemotherapy may be designed.3 A rapid colorimetric assay based on the ability of viable cells to reduce a tetrazolium-based compound (MTT) to a blue formazan product is an increasingly used assay for testing in vitro chemosensitivity of leukemic samples.2,3,4,5,7,8 MTT assay provides a good correlation between in vitro resistance to cytotoxic drugs and prognosis in childhood leukemia.1
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Karakaş, Z., Ağaoğlu, L., Erdem, S., Anak, S., Hacıbektaşoğlu, A., Gedikoğlu, G. (1999). MTT Assay for Drug Resistance in Childhood Acute Leukemia and Effect of Cyclosporin and Interferon. In: Kaspers, G.J.L., Pieters, R., Veerman, A.J.P. (eds) Drug Resistance in Leukemia and Lymphoma III. Advances in Experimental Medicine and Biology, vol 457. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4811-9_49
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DOI: https://doi.org/10.1007/978-1-4615-4811-9_49
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