Abstract
Gatal is expressed from either one of two alternative promoters, the erythroid (or proximal with respect to the AUG) and the testis (or distal with respect to the AUG) promoter, both of which are utilized by erythroid cells. We have determined by specific RT-PCRs the relative levels of the two Gatal transcripts during the differentiation of erythroid precursors purified from the spleen of phenylhydrazine (PHZ)-induced anemic mice. Day 1 PHZ-spleen cells are mainly composed by erythroid precursors that synchronously progress in vivo, and in vitro in the presence of ery-thropoietin (EPO), to mature erythroblasts. The total levels of Gatal expression increased by 5–8-fold during in vivo and in vitro differentiation of PHZ-cells. Such increase was correlated with increased levels of the distal, but not of the proximal Gatal transcripts. The day 1 PHZ-cells which survived 18 hours in culture in the absence of EPO, were benzidine-negative and expressed detectable levels of the distal, but not of the proximal, Gatal transcripts. When re-exposed to EPO, these cells activated the expression of the globin genes within 2 hrs. The existence of an erythroid precursor which expresses only distal Gatal transcripts, and the fact that the increase of Gatal expression observed during erythroid differentiation is due to increased levels of transcripts from the distal promoter, suggest that regulation of the expression of Gatal from its distal promoter is an important step in the erythroid differentiation program.
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© 1999 Springer Science+Business Media New York
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Vannucchi, A.M., Linari, S., Migliaccio, A.R. (1999). Expression Of Distal gatal Transcripts in Erythroid Cells. In: Abraham, N.G., Tabilio, A., Martelli, M., Asano, S., Donfrancesco, A. (eds) Molecular Biology of Hematopoiesis 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4797-6_31
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DOI: https://doi.org/10.1007/978-1-4615-4797-6_31
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