Abstract
We have developed a novel cell sorter, the dipole magnet flow sorter (DMFS) which separates cells into multiple fractions according to theirsurface marker expression, as determined by flow cytometry. A narrow stream of cells is introduced into a sheath of carrier fluid in a rectangular channel while subjected to a perpendicular magnetic force. The special design of the pole pieces ensures a constant magnetic force acting on the cells in the separation area. Cells are spread across the flow in relation to their magnetophoretic mobilities. Separation is achieved by control of the positions of the stream boundaries dividing outlet fractions. CD8 T-lymphocytes labeled with primary antibody-FITC conjugate and anti-FITC-magnetic colloid is the chosen cell system. Flow cytometry analysis shows that a four-fold increase in total marker number per cell is observed over the range of recovered fractions. We believe that this type of separation is applicable to any cells in suspension for which a suitable antibody exists, and — due to the comparatively gentle nature of the process—is particularly suitable for the sorting of fragile cells.
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Moore, L.R., Zborowski, M., Sun, L., Chalmers, J.J. (1999). Lymphocyte Fractionation in a Magnetic Flow Cell Sorter. In: Abraham, N.G., Tabilio, A., Martelli, M., Asano, S., Donfrancesco, A. (eds) Molecular Biology of Hematopoiesis 6. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4797-6_28
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DOI: https://doi.org/10.1007/978-1-4615-4797-6_28
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