Abstract
A new biotest system for ecotoxicological evaluation of soil pollutants was developed, using the symbiosis of Rhizobium meliloti strains with Medicago sativa cultivars, with the number of nodules formed as effect parameter. The development of the test system is described. Soil contaminating substances such as heavy metals, nitroaromatic compounds and polycyclic aromatic hydrocarbons (PAHs), reduced nodulation without damage to the plants and bacteria as separate units at distinct concentration levels. A dose-responsive decrease in nodulation was especially found after application of cadmium ions, sodium salts of arsenate and arsenite, arsenic pentoxide, and lead nitrate. The dose response curves were used to calculate EC values. EC50 values (50% inhibition) for cadmium compounds range from 1.5 to 9.5.tM. Arsenic compounds result in EC50 from 2.6 to 20.1 μM. Sensitivity, reproducibility, and reliability of this test system is discussed and compared to other established biotests such as the LumisTox-test with the example of nitroaromatic compounds such as TNT, 2,4-DNT, 2,6-DNT, and 4-ADNT.
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References
Chino M 1981. In Heavy metal pollution in soils of Japan eds. K Kitagishi and I Yamane, pp 65–80, Japan Sci. Soc. Press, Tokyo.
Codina JC, Perez-Garcia A, Romero P, and de Vicente A 1993. A comparison of microbial bioassays for the detection of metal toxicity, Arch. Environ. Contam. Toxicol. 25, 250–254.
DIN 38 412 Teil 34 1991. Bestimmung der Hemmwirkung von Abwasser auf die Lichtemission von Photobacterium phosphoreum—Leuchtbakterien-Abwassertest mit konservierten Bakterien (L34). Testverfahren mit Wasserorganismen (Gruppe L).
Freiberg C, Fellay R, Bairoch A, Broughton WJ, Rosenthal A, and Perret X 1997. Molecular basis of symbiosis between Rhizobium and legumes, Nature 387,394–401.
Hennecke H 1997. Rhizobial respiration to support symbiotic nitrogen fixation. In Biological Nitrogen Fixation of the 21st Century. Eds. C Elmerich, A Kondorosi and WE Newton, pp 183–484, Kluwer Academic Publishers, Dordrecht.
Landberg T and Greger M 1994. Influence of selenium on uptake and toxicity of copper and cadmium in pea (Pisum sativum) and wheat (Triticum aestivum), Physiol. Plant. 90, 637–644.
Liebig GF, Bradford GR, and Vanselow AP 1959. Soil Sci. 88, 342–348.
Mavingui P, Flores M, Romero D, Martinez-Romero E, and Palacios R 1997. Generation of Rhizobium strains with improved symbiotic properties by random DNA amplification (RDA), Nature Biotechnology 15, 564–569.
Neumann H, Bode-Kirchhoff A, Madeheim A, and Wetzel A 1998. Toxicity testing of heavy metals with the Rhizobium-legume symbiosis with high sensitivity to cadmium and arsenic compounds, ESPR 5 (1), 28–36.
Phillips DA and Streit WR 1997. Applying plant-microbe signalling concepts to alfalfa: Roles for secondary metabolites. In Biotechnology and the Improvement of Forage Legumes eds. BD McKersie and DCW Brown, pp 319–342, CAB International, Wallingford.
Pühler A 1997. Signalling processes in root nodule organogenesis: Introductory remarks. In Biological Nitrogen Fixation of the 21st Century, eds. C Elmerich, A Kondorosi and WE Newton, pp 183–184, Kluwer Academic Publishers, Dordrecht.
Vance CP 1997. Nitrogen fixation capacity. In Biotechnology and the Improvement of Forage Legumes, eds. BD McKersie and DCW Brown, pp 375–407, CAB International, Wallingford.
Werner D 1992. Symbiosis of Plants and Microbes, 2nd Ed. Chapman and Hall, London. 390 p.
Wetzel A, Brandt S, Görge E, and Werner D 1991. A new plant test system for ecotoxicological evaluation of polycyclic aromatic hydrocarbons—The legume-root-nodule symbiosis as effect parameter, Proceedings of the International Symposium Environmental Biotechnology II, pp 687–689, Ostende.
Wetzel A, Parniske M, and Werner D 1995. The pleiotrophic effects of fluoranthene on anthocyanin synthesis and nodulation of Medicago sativa is reversed by the plant flavone luteolin, Bull. Environ. Contam. Toxicol. 54, 633–639.
Wetzel A and Werner D 1995. Ecotoxicological evaluation of contaminated soil using the legume root nodule symbiosis as effect parameter, Environ. Toxicol. and Water Quality 10,127–133.
Wetzel A 1998. Advances in biomonitoring: sensitivity and reliability in PAH-contaminated soil. In Environmental Biomonitoring: The Bio-technology-Ecotoxicology Interface, eds. J Lynch and Wiseman, Cambridge University Press, Cambridge.
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© 1999 Springer Science+Business Media New York
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Werner, D., Neumann, H., Scheidemann, P., Bode-Kirchhoff, A., Wetzel, A. (1999). Bioassays for Soil Contamination Using the Legume Root Nodule Symbiosis. In: Martĺnez, E., Hernández, G. (eds) Highlights of Nitrogen Fixation Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4795-2_46
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DOI: https://doi.org/10.1007/978-1-4615-4795-2_46
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