Abstract
The specific endophytic association between Acetobacter diazotrophicus and sugarcane may represent a beneficial symbiosis with the bacterial partner providing fixed N and/or plant growth promoting factors such as auxin. Our goals are to identify and characterize the genes of A. diazotrophicus necessary for nitrogen fixation and effective symbiosis. In summary, we have isolated a gene cluster including nifAB HDK ENXQ USVW, fixABCX in one region of the A. diazotrophicus genome. Also, two other genes, modD (molybdenum transporter protein) and mcpA (methyl-accepting chemotaxis protein) were located downstream of fixABCX. The mcpA gene product is involved in responses to extracellular chemotactic signals, which may play an important role in plant-microbe interactions. A mcpA mutant of A. diazotrophicus lost chemotaxis toward a wide range of attractant stimuli and will be used to determine whether mcpA + bacteria have a competitive advantage with respect to cell survival and colonization of sugarcane.
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References
Barbara R, Thomas H, Istban F 1985. Strain specific chemotaxis of Azospirillum spp. J. Bacteriol 162,190–195
Dean D, Jacobson MR 1992. Biochemical genetics of nitrogenase In Biological Nitrogen Fixation. Stacey G, Evans HJ, Burris R, eds. Chapman & Hall, New York, pp 763–834
Jacobson MR, Brigle KE, Bennett L, Setterquist R, Wilson M, Cash V, Beynon J, Newton W, and Dean D 1989. Physical and genetic map of the major nif gene cluster from Azotobacter vinelandü. J Bacteriol 171, 1017–1027
Masepohl B, Klipp W 1996. Organization and regulation of genes encoding the molybdenum nitrogenase and the alternative nitrogenase in Rhodobacter capsulatus. Arch Microbiol 165, 80–90
Sevilla M, Meletzus D, Teixeira K, Lee S, Nutakki A, Baldani I, Kennedy C 1997. Analysis of nif and regulatory genes in Acetobacter diazotrophicus. Soil Biol Biochem 29, 871–874
Sevilla M, Oliveira A de, Baldani I, and Kennedy C 1998. Contribution of the bacterial endophyte Acetobacter diazotrophicus to sugarcane nutrition: a preliminary study. Symbiosis 25,181–191
Stephan MP, Oliveria M, Teixeira G, Martinez-Drets G, Dobereiner J 1991. Physiology and dinitrogen fixation of Acetobacter diazotrophicus. FEMS Microbiology Letters 77, 67–72
Wang G, Angermuller S, Klipp W 1993. Characterization of Rhodobacter capapsulatus genes encoding molyb-denum transport system and putative molybdenum-pterin-binding proteins. J Bacteriol 175, 3031–3042
Ward MJ, Harrison DM, Ebner MJ, Armitage JP 1995. Identification of a methyl-accepting chemotaxis protein in Rhodobacter sphaeroides. Mol. Biol. 17, 357–366
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© 1999 Springer Science+Business Media New York
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Lee, S., Sevilla, M., Meletzus, D., Texeira, K., Baldani, I., Kennedy, C. (1999). Analysis of Nitrogen Fixation and Regulatory Genes in the Sugarcane Endophyte Acetobacter Diazotrophicus . In: Martĺnez, E., Hernández, G. (eds) Highlights of Nitrogen Fixation Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4795-2_43
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DOI: https://doi.org/10.1007/978-1-4615-4795-2_43
Publisher Name: Springer, Boston, MA
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