Abstract
fixT was recently identified as a repressor of nitrogen fixation gene expression in S. meliloti. Here we report on the characterization of the mode of action of FixT. We propose that FixT primarily counteracts the activity of the FixLJ two-component system, by decreasing its phosphorylation state. Specifically, we have found that FixT is an inhibitor of the sensor haemoprotein kinase FixL.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Foussard M, Garnerone AM, Ni F, Soupène E, Boistard P, and Batut J 1997. Negative autoregulation of the Rhizobium meliloti fixK gene is indirect and requires a newly identified regulator, FixT. Mol. Microbiol. 25, 27–37.
Galinier A, Garnerone AM, Reyrat JM, Kahn D, Batut J, and Boistard P 1994. Phosphorylation of the Rhizobium meliloti FixJ protein induces its binding to a compound regulatory region at the fixK promoter. J. Biol. Chem. 269, 23784–23789.
Gilles-Gonzalez MA and Gonzalez G 1993. Regulation of the kinase activity of heme protein FixL from the two-component system FixL/FixJ of Rhizobium meliloti. J. Biol. Chem. 268, 16293–16297.
Lukat GS, McCleary WR, Stock AM, and Stock JB 1992. Phosphorylation of bacterial response regulator proteins by low molecular weight phospho-donors. Proc. Natl. Acad. Sci. USA 89, 718–722.
Perego M, Hanstein CG, Welsh KM, Djavakhishvili T, Glaser P, and Hoch JA 1994. Multiple protein aspar-tate phosphatases provide a mechanism for the integration of diverse signals in the control of development in Bacillus subtilis. Cell 79,1047–10555.
Reyrat JM, David M, Blonski C, Boistard P, and Batut J 1993. Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes. J. Bacteriol. 175, 6867–6872.
Wang L, Grau R, Perego M, and Hoch JA 1997. A novel histidine kinase inhibitor regulating development in Bacillus subtilis. Genes and Dev. 11, 2569–2579.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Garnerone, AM., Foussard, M., Boistard, P., Batut, J. (1999). Mode of Action of the FixT Repressor Protein of Sinorhizobium Meliloti . In: Martĺnez, E., Hernández, G. (eds) Highlights of Nitrogen Fixation Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4795-2_39
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4795-2_39
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7172-4
Online ISBN: 978-1-4615-4795-2
eBook Packages: Springer Book Archive