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The Griffonia simplicifolia I - B4 Isolectin

A Probe for α-D-Galactosyl End Groups

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α-Gal and Anti-Gal

Part of the book series: Subcellular Biochemistry ((SCBI,volume 32))

Abstract

Lectins are a heterogeneous group of proteins with the common property of recognizing carbohydrate molecules, often in a very specific manner. Although first discovered in plant seeds, they also have been found in other vegetative tissues of the plant world (leaves, stems, bark, roots, tubers, etc.). In recent years, lectins have been isolated from microorganisms, insects, Crustacea, rodents, and most recently from various tissues of primates where they appear to be involved in cellular adhesion, clearance of glycoproteins from the circulating system, cell-cell recognition, etc.

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References

  • Bankston, P. W., Porter, G. A., Milici, A. J.,and Palade, G. E., 1991, Differential and specific labeling of epithelial and vascular endothelial cells of the rat lung by Lycopersicon esculentum and Griffonia simplicifolia I lectins, Eur. J. Cell Biol. 54: 187–195.

    PubMed  CAS  Google Scholar 

  • Blake, D. A. and Goldstein, I. J., 1980. Resolution of nucleotide sugars and oligosaccharides by lectin affinity chromatography. Anal. Biochem. 102: 103–109.

    Article  PubMed  CAS  Google Scholar 

  • Blake, D. A. and Goldstein, I. J., 1982. Resolution of carbohydrates by lectin affinity chromatography. Meth. Enzymol. 83: 127–132.

    Article  PubMed  CAS  Google Scholar 

  • Brabec, R. K., Peters, B. P., Bernstein, I. A., Gray. R. H., and Goldstein, I. J., 1980, Differential lectin binding to cellular membranes in the epidermis of the newborn rat, Proc. Natl. Acad. Sci. USA 77: 477–479.

    Article  PubMed  CAS  Google Scholar 

  • Castronovo, V., Colin, C. Parent, B., Foidart, J.-M., Lambotte. R., and Mahieu. P., 1989, Possible role of human natural anti-Gal antibodies in the natural antitumor defense system.J. Nail. Cancer inst. 81:212–216.

    Article  CAS  Google Scholar 

  • Cho. S. K., and Cummings, R. D., 1997. A soluble form of α-1.3 galactosyltransferase within cells to galactosylate glycoproteins. J. Biol. Chem. 272: 13622–13628.

    Article  PubMed  CAS  Google Scholar 

  • de Boeck, H., Loontiens, F. G., Delmotte, E. M. and de Bruyne, C. K., 1981. The use of 4-methylumbelliferyl glycosides in binding studies with the lectins BS I-A4, BS I-B4 and BS II from Bandeiraea (Griffonia) simplicifolia, FEBS Lett. 126: 227–230.

    Article  Google Scholar 

  • Delmotte, F. M. and Goldstein, I. J., 1980. Improved procedure for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea II lectin by affinity chromatography, Eur. J. Biochem. 112:219–223.

    Article  PubMed  CAS  Google Scholar 

  • Eckhardt, A. E. and Goldstein, I. J. 1983a, Occurrences of α-D-galactosyl-containing glycoproteins on Ehrlich tumor cell membranes. Biochemistry 22: 5280–5289.

    Article  PubMed  CAS  Google Scholar 

  • Eckhardt, A. E. and Goldstein, l. J., 1983b. Isolation and characterization of a family of α-D-galactosyl-containing glycopeptides from Ehrlich ascites tumor cells. Biochemistry 22: 5290–5297.

    Article  PubMed  CAS  Google Scholar 

  • Eckhardt, A. E., Goldstein. I. J., and Nelsestuen. G L. 1985. Bovine prothrombin does not contain α-D-galactopyranosyl groups, Arch. Biochem. Biophys. 169: 635–636.

    Google Scholar 

  • Eckhardt, A. E., Malone. B. N., and Goldstein, I. J., 1982, Inhibition of Ehrlich ascites tumor cell growth by Griffonia simplicifolia I lectin in vivo. Cancer Res. 42. 2977–2979.

    PubMed  CAS  Google Scholar 

  • Edge, A. S. B. and Spiro, R. G., 1985, Thyroid cell surface glycoproteins. Nature and deposition of carbohydrate units and evaluation of their blood group I activity, J. Biol. Chem. 260: 1532–1538.

    Google Scholar 

  • Edge, A. S. B. and Spiro. R. G., 1997, Structure of the O-linked oligosaccharides from a major thyroid cell surface glycoprotem. Arch. Biochem. Biophys. 343(l): 73–80.

    Article  PubMed  CAS  Google Scholar 

  • Faraggiana, T., Churg, J. Grishman, E., Strauss, L., Prado, A., Bishop, D. F., Schuchman, E., and Desnick, R. J., 1982, Light-and electron-microscope histochemistry of Fabry’s Disease. Am. J. Pathol. 103: 247–262.

    Google Scholar 

  • Faraggiana, T., Crescenze. A., and Marinozzi. V., 1989. Presence of an α-galactolipid on the cell surfaces of endothelial cells of human kidney. Histochem. J. 21: 235–240.

    Article  PubMed  CAS  Google Scholar 

  • Galili, U., 1993, Interaction of the natural anti-Gal antibody with α-galactosyl epitopes: a major obstacle for xenotransplantation in humans, Immunol. Today 14: 480–482.

    Article  PubMed  CAS  Google Scholar 

  • Galili, U., Clark, M R., Shohet, S. B., Buehler, J., and Macher, B A, 1987, Evolutionary relationship between the natural anti-Gal antibody and the Galα 1,3Gal epitope in primates, Proc. Natl. Acad. Sci. USA 84: 1369–1373.

    Article  PubMed  CAS  Google Scholar 

  • Galili, U., Macher, B A, Buehler, J. and Shohet, S. B., 1985, Human natural anti-α-galactosyl IgG. II. The specific recognition of α-(l→3)-linked galactose residues, J. Exp. Med. 162: 573–582.

    Article  PubMed  CAS  Google Scholar 

  • Galili, U., Shohet, S. B., Kobrin, E., Stults, C. L., and Macher, B. A., 1988, Man, apes, and old world monkeys differ from other mammals in the expression of α-galactosyl epitopes on nucleated cells, J. Biol. Chem. 263: 17755–17762.

    PubMed  CAS  Google Scholar 

  • Goldstein, I. J., Blake, D. A., Ebisu, S., Williams, T. J. and Murphy, L. A., 1981, Carbohydrate binding studies on the Bandeiraea simplicifolia I isolectins. Lectins which are mono-, di-, tri-, and tetravalent for N-acetyl-D-galactosamine, J. Biol. Chem. 256: 3890–3893.

    PubMed  CAS  Google Scholar 

  • Goldstein, I. J., Winter, H. C., and Poretz, R. D., 1997, Plant lectins: tools for the study of complex carbohydrates in: Glycoproteins II, J. Montreuil, J. F. G. Vliegenthart, and H. Schachter, eds., Elsevier, Amsterdam, pp. 403–474.

    Chapter  Google Scholar 

  • Gorelik, E., Duty, L., Anaraki, F.,and Galili, U., 1995, Alteration of cell surface carbohydrates and inhibition of metastatic property of murine melanomas by α-1,3 galactotransferase gene transfection, Cancer Res. 55: 4168–4173.

    PubMed  CAS  Google Scholar 

  • Hayes, C. E. and Goldstein, I. J., 1974, An α-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization, J. Biol. Chem. 249: 1904–1914.

    PubMed  CAS  Google Scholar 

  • Hayes, C. E. and Goldstein, I. J., 1975, Equilibrium dialysis and cell binding studies on Bandeiraea simplicifolia lectin, J. Biol. Chem. 250: 6837–6840.

    PubMed  CAS  Google Scholar 

  • Horejsi, V. and Kocourek, J., 1973, Studies on phytohemagglutinins XII. O-Glycosyl polyacrylamide gels for affinity chromatography of phytohemagglutinins. Biochim. Biophys. Acta. 297: 346–351.

    Article  PubMed  CAS  Google Scholar 

  • Ihida, K., Tsuyama, S., and Murata, F., 1990. The use of Griffonia simplicifolia agglutinin-I B4 in staining the developing rat fundic gland, Acta. Hislochem. Cytochem. 23: 475–86.

    Article  CAS  Google Scholar 

  • Ito, N., Imai, S., Haga, S., Nagaike, C., Morimura, Y., and Hatake, K., 1996, Localization of binding of Ulex europeus I, Helix pomata and Griffonia simplicifolia I-B4 lectins and analysis of their backbone structures by several glycosidases and poly-N-acetyllactosamine specific lectins in human breast carcinomas, Histochem. Cell Biol. 106: 331–339.

    PubMed  CAS  Google Scholar 

  • Ito, N., Nagaike, C., Morimura, Y., and Hatake, H., 1997. Estimation and comparison of the content of blood group B antigen in selected human tissues by microphotomeric quantitation of Griffonia simplicifolia agglutinin I-B4 staining with or without prior α-galactosidase treatment, Histol. Histopathol. 12: 415–424.

    PubMed  CAS  Google Scholar 

  • Kaifu, R., Plantefaber, L. C., and Goldstein, I. J., 1985, 2-substituted methyl α-D-galactopyranosides: synthesis and binding affinity for the A and B subunits of the Griffonia simplicifolia I isolectins, Carbohydr. Res. 140: 37–49.

    Article  PubMed  CAS  Google Scholar 

  • Kusai, K., Yamatnoto, K., Konami, Y., and Osawa. T., 1991, cDNA cloning and expression of Bauhinia purpurea lectin, J. Biochem. (Tokyo) 109: 899–903.

    Google Scholar 

  • Koite, C., Katayama, A., Kadomatsu, K., Muramatsu, T., Hiraiwa, N., Kannagi, R., Nakashima, I., Yokoyama, I., Takagi, H., 1997, Direct gene replacement of the mouse α(1,3)-galactosyltransferase gene with human α(1,2)-fucosyltransferase gene: converting α-galactosyl epitopes into H antigens, Xenotransplantalion 4: 147–153.

    Article  Google Scholar 

  • Knibbs, R. N., Perini, F. and Goldstein, I. J., 1989, Structure of the major concanavalin A-reactiveoligosaccharides of the extracellular matrix component laminin. Biochemistry 28: 6379–6392.

    Article  PubMed  CAS  Google Scholar 

  • Lamb, J. E. and Goldstein, I. J., 1984. A structural comparison of the A and B subunits of Griffonia simplicifolia I isolectins, Arch. Biochem. Biophvs. 229: 15–26.

    Article  CAS  Google Scholar 

  • Liener, I. E., Sharon, N., and Goldstein, I. J., eds., 1986, The lectins: properties, function and application in biology and medicine, pp. 1–600.

    Google Scholar 

  • Lis, H., and Sharon, N., 1998, Lectins: carbohydrate specific proteins that mediate cellular recognition, Chem. Rev. in press.

    Google Scholar 

  • Lönngren, J. and Goldstein, I. J., 1976, Chemical modification studies on the α-D-galactopyranosyl binding lectin from the seeds of Bandeiraea simplicifolia, Biochim. Biophys. Acta. 439: 160–166.

    Article  Google Scholar 

  • Lönngren, J., Goldstein, I. J., and Bywater, R., 1976. Cross-linked guaran: a versatile immunosorbent for D-galactopyranosyl-binding lectins, FEBS Lett. 68: 31–34.

    Article  PubMed  Google Scholar 

  • Lönngren, J., Goldstein, I. J., and Zand, R., 1976. Circular dichroism studies on the α-D-galactopyranosyl binding lectin isolated from the seeds of Bandeiraea simplicifolia, Biochemistry 15: 436–440.

    Google Scholar 

  • Maddox, D. E., Shibata, S., and Goldstein, I. J., 1982a, Stimulated macrophages express a new glycoprotein receptor reactive with Griffonia simplicifolia I-B4 isolectin, Proc. Natl. Acad. Sci. USA 79: 166–170.

    Article  PubMed  CAS  Google Scholar 

  • Maddox, D. E., Goldstein, I. J., and Lobuglio, A. E., 1982b, Griffonia simplicifolia I lectin mediates macrophage-induced cytotoxicity against Ehrlich ascites tumor, Cell. Immunol. 71:202–207.

    Article  PubMed  CAS  Google Scholar 

  • Mäkelä, O., 1957, Studies on hemagglutinins of Leguminosae seeds, Ann. Med. Exp. Biol. Fenn. 35: Suppl. 11, 1–133.

    PubMed  Google Scholar 

  • Mäkelä, O. and Mäkelä, P., 1956, Some new blood groups specific phytoagglutinins, Ann. Med. Exp. Biol. Fenn. 34: 402–404.

    PubMed  Google Scholar 

  • Mäkelä, O., Mäkelä, P., and Krüpe. M., 1959, Zur Spezifität der Anti-B-Phythämagglutinine, Z. Immunitasetsforsch. Exp. Ther. 117: 220–229.

    Google Scholar 

  • Murphy, L. A. and Goldstein. I. J., 1977. Five α-D-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds, J. Biol. Chem. 252: 4739–4742.

    PubMed  CAS  Google Scholar 

  • Murphy, L. A. and Goldstein. I. J., 1979. Physical-chemical characterization and carbohydrate-binding activity of the A and B subunits of the Bandeiraea simplicifolia I isolectins, Biochemistry 18: 4999–5005.

    Article  PubMed  CAS  Google Scholar 

  • Nelsestuen, G.L. and Sattie, J. W., 1972, The carbohydrate of bovine prothrombin. Partial structural determination demonstrating the presence of α-galactose residues, J. Biol. Chem. 247: 6096–6102.

    PubMed  CAS  Google Scholar 

  • Okada, Y. and Spiro, R. G., 1980, Isolation and characterization of three major glycoproteins from thyroid plasma membranes, J. Biol. Chem. 255: 8865–8872.

    PubMed  CAS  Google Scholar 

  • Oppenheim, J. D., Amin, A. R., and Thorbeck, C. J., 1990, A rapid one-step purification procedure for murine igD based on the specific affinity of Bandeiraea (Griffonia) simplicifolia I for N-linked carbohydrates on IgD. J. Immunol. Meth. 130: 243–250.

    Article  CAS  Google Scholar 

  • Peters, B.P. and Goldstein, l.J.,1979, The use of fluorescein-conjugated Bandeiraea simplicifolia B4 isolectin as a histochemical reagent for the detection of α-D-galactosyl groups, Exp. Cell Res. 120:321–334.

    Article  PubMed  CAS  Google Scholar 

  • Petryniak, J., Varani, J., Ervin, P. R., and Goldstein. I. J., 1991. Differential expression of glycoproteins containing α-D-galactosyl groups on normal human breast epithelial cells and MCF-7 human breast carcinoma cells, Cancer Lett. 60: 59–65.

    Article  PubMed  CAS  Google Scholar 

  • Petryniak, J., Huard, T. K., and Goldstein. I. J., 1992, α-D-Galactose-bearing glycoproteins on the surface of stimulated murine peritoneal macrophages. Biochemical and immunochemical characterization of purified glycoproteins. Eur. J. Biochem. 206: 197–207.

    Article  PubMed  CAS  Google Scholar 

  • Rao, C.N., Goldstein. I. J.,and Liotta, L. A., 1983. Lectin-binding domains on laminin, Arch. Biochem. Biophys. 227: 1 18–124.

    Article  Google Scholar 

  • Ross, T. T., Hayes, C. E., and Goldstein. I. J., 1976. Carbohydrate-binding properties of an immobilized α-D-galactopyranosyl binding protein (lectin) from the seeds of Bandeiraea simplicifolia. Carbohydr. Res. 47: 91–97.

    Article  PubMed  CAS  Google Scholar 

  • Roth, J. and Goldstein, I. J., 1995. Subcellular distribution of terminal α-D and β-D galactosyl residues in Ehrlich tumor cells studied by lectin-gold techniques, Glycoconjugate. J. 12: 142–149.

    Article  CAS  Google Scholar 

  • Shibata, S., Peters, B.P. Roberts, D. D., Goldstein, I. J. and Liotta, L A, 1982, Isolation of laminin by affinity chromatography on Griffonia simplicifolia I lectin, FEBS Letts. 42(2): 194–1

    Article  Google Scholar 

  • Shinkel, T. A., Chen, C.-G., Solvaris, E., Henion. T. R., Barlow. H., Galili, U., Pearse, M. J., and D’Apice, A. J. F., 1997, Changes in cell surface glycosylation in α-1,3 galactosyl-transferase knockout and α-1.2-fucosyltransferase transgenic mice. Transplantation 64: 197–204.

    Article  PubMed  CAS  Google Scholar 

  • Tearle, R. G., Tange. M. J., Zannettino. Z. L., Katerelos, M., Shinkel, T. A., Van Denderen. B. J. W., Lonie, A. J., Lyons, I., Nottle, M. B., Cox. T., Becker, C. Peura. A. M., Wigley, P. L., Crawford, R. J., Robins, A. J., Pearse, M. J., and D’Apice. A. J. F., 1996, The α 1,3 galactosyltransferase knockout mouse: Implications for xenotransplantation. Transplantation 61: 13–19.

    Article  PubMed  CAS  Google Scholar 

  • Thall, A. D. and Galili, U., 1990, Distribution of Galα→3Galβ1→4GlcNAc residues on secreted mammalian glycoproteins (thyroglobulin, fibrinogen, and immunoglobulin G) as measured by a sensitive solid-phase radioimmunoassay, Biochemistry 29: 3959–3965.

    Article  PubMed  CAS  Google Scholar 

  • Thall, A. D., Maly, P., and Lowe, J. B., 1995, Oocyte gal α 1,3 gal epitopes implicated in sperm adhesion to the zona pellucida glycoprotein ZP3 are not required for fertilization in the mouse, J. Biol. Chem. 15: 21437–21440.

    Google Scholar 

  • Vanhove, B., Goret, F., Soulillou, J.-P., and Pourcel, C., 1997, Porcine α-1,3 galactosyl-transferase: tissue specific and regulated expression of splicing isoforms, Biochim. Biophys. Acta. 1356: 1–11.

    Article  PubMed  CAS  Google Scholar 

  • Watier, H., Guillaumin, J.-M., Piller, F., Lacord, M., Thibault, G., Lebranchu, Y., Monsigny, M., and Bardos, P., 1996, Removal of terminal α-galactosyl residues from xenogenic porcine endothelial cells, Transplantation 62: 105–113.

    Article  PubMed  CAS  Google Scholar 

  • Williams, T. J., Plessas, N. R., and Goldstein, I. J. and Lönngren, J., 1979, A new class of model glycolipids: synthesis, characterization, and interaction with lectins, Arch. Biochem. Biophys. 195: 145–151.

    Article  PubMed  CAS  Google Scholar 

  • Wood, C., Kabat, E. A., Murphy L. A., and Goldstein, I. J., 1979. Immunochemical studies of the combining sites of the two isolectins A4 and B4 isolated from Bandeiraea simplicifolia. Arch. Biochem. Biophys. 198: 1–11.

    Article  PubMed  CAS  Google Scholar 

  • Wu, A. M., Song, S.-C., Wu, J. H., and Kabat, E. A., 1995. Affinity of Bandeiraea (Griffonia) simplicifolia lectin I, isolectin B4 for Galα1→4Gal ligand, Biochem. Biophys. Res. Commun. 216:814–820.

    Article  PubMed  CAS  Google Scholar 

  • Young, N.M., and Oomen, R. P., 1992, Analysis of sequence variation among legume lectins. A ring of hypervariable residues forms the perimeter of the carbohydrate-binding site, J. Mol. Biol. 228: 924–934.

    Article  PubMed  CAS  Google Scholar 

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Goldstein, I.J., Winter, H.G. (1999). The Griffonia simplicifolia I - B4 Isolectin. In: Galili, U., Avila, J.L. (eds) α-Gal and Anti-Gal. Subcellular Biochemistry, vol 32. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4771-6_6

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  • DOI: https://doi.org/10.1007/978-1-4615-4771-6_6

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