Summary
Often when a project is initiated plant cell cultures produce low levels of a desired compound. For the project to move to a commercial level, time-dependent volumetric yields (mg.product/L-day) must typically be increased several orders of magnitude. Typically, no single growth enhancing strategy will give such a large increase, but in many cases the simultaneous application of multiple-strategies results in a synergistic interaction and the large increases necessary to bring yields in a plant cell culture process to a useful level. Yield improvement strategies include selection of stable, high-yielding variants; manipulation of medium components and/or gas phase composition; metabolic manipulation through gene manipulation or use of inhibitors; precursor feeding; use of elicitors;in situproduct removal; and use of immobilization or more differentiated or organized plant material.
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Shuler, M.L. (1999). Overview of Yield Improvement Strategies for Secondary Metabolite Production in Plant Cell Culture. In: Fu, TJ., Singh, G., Curtis, W.R. (eds) Plant Cell and Tissue Culture for the Production of Food Ingredients. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4753-2_7
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DOI: https://doi.org/10.1007/978-1-4615-4753-2_7
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