Skip to main content

Production of Betalains by Hairy Root Cultures of Beta Vulgaris L

  • Chapter

Summary

Hairy root cultures of Beta vulgaris L. produce betalains similar to roots in planta. These root cultures have shown stability in biomass and pigment production for several generations. The concentration of betalains in these root cultures is affected by various physical and chemical factors. Some of the factors which can be used for increasing the productivity of these root cultures are light, media constituents and elicitors. Thus, beet hairy root cultures can serve as a model system for studying betalain biosynthesis and also have potential for development of large-scale production of betalains for use as a food color.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bianco-Colomas, J.; Hugues, M. Establishment and characterization of a betacyanin producing cell line of Amaranthus tricolor: inductive effects of light and cytokinin. Journal of Plant Physiology 1990, 136, 734–739.

    Article  CAS  Google Scholar 

  • Bohm, H.; Rink, E. Betalains. In Cell Culture and Somatic Cell Genetics of Plants. Vol 5; Vasil, I. K., Ed.; Academic Press: New York, 1988; pp 449–463.

    Google Scholar 

  • Chilton, M. D.; Tepfer, D.; Petit, A.; David, D.; Casse-Delbart, F; Tempe, J. Agrobacterium rhizogenes inserts T-DNA into the genomes of host plant cells. Nature 1982, 295, 432–434.

    Article  CAS  Google Scholar 

  • Constabel, F.; Nassif-Makki, H. Betalainbildung in Beta calluskrultiren. Ber. Dtsch. Bot. Ges. 1971, 84, 629–636.

    CAS  Google Scholar 

  • Dilorio, A. A.; Chetham, R. D.; Weathers, P. J. Growth of transformed root cultures in a nutrient mist bioreactor: reactor performance and evaluation. Applied Microbiology and Biotechnology 1992, 37, 457–462.

    Article  Google Scholar 

  • Eilert U. Elicitation: methodology and aspects of application. In Cell Culture and Somatic Cell Genetics of Plants. Vol.4.; Vasil, I. K., Ed.; Academic Press: New York, 1987; pp 153–195.

    Google Scholar 

  • Flores, H. E. Use of plant cells and organ culture in the production of biological chemicals. In Biotechnology In Agricultural Chemistry. LeBaron, H. M., Mumma, R. O., Honeycutt, R. C., Duesing, J. H., Phillips, J. F., Haas, M. J., Eds.; American Chemical Society: Washington, D.C., 1987; pp 66–86.

    Chapter  Google Scholar 

  • Girod, P.A.; Zryd, J. P. Studies of the regulation of betalain biosynthesis in red beet cells in cultures: competence for light induction. Experientia 1986, 42, 653–654.

    Google Scholar 

  • Girod, P.A.; Zryd, J. P. Clonal variability and light induction of betalain biosynthesis in red beet cell cultures. Plant Cell Reports 1987, 6, 27–30.

    Article  CAS  Google Scholar 

  • Girod, P. A; Zryd, J. P. Secondary metabolism in cultured beet (Beta vulgaris, L.) cells: differential regulation of betaxanthin and betacyanin biogenesis. Plant Cell Tissue and Organ Culture 1991, 25, 1–12.

    Article  CAS  Google Scholar 

  • Hamill, J. D.; Parr, A. J.; Robins, R. J.; Rhodes, M. J. C. Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Reports 1986, 5, 111–114.

    Article  CAS  Google Scholar 

  • Leathers, R. R.; Davin, C.; Zryd, J. P. Betalain producing cell cultures of Beta vulgaris L. var. bikores monogerm (red beet). In Vitro Cell Developmental Biology 1992, 28, 39–45.

    Article  Google Scholar 

  • Mukandan, U.; Carvalho, E., Curtis, W. R. Growth and pigment production by hairy root cultures of Beta vulgaris L. in a bubble column reactor. Biotechnology Letters 1998, in press.

    Google Scholar 

  • Nilsson, T. Studies into the pigments in beet root. Lantbrukshogskolans Annaler 1970, 36, 179–219.

    CAS  Google Scholar 

  • Piatelli, M. Betalains: chemistry and biosynthesis and chemical taxonomy. In The Biochemistry of Plants. Vol. 7; Conn, E. E., Ed.; Academic Press: New York, 1976; pp 557–575.

    Google Scholar 

  • Taya, M.; Mine, K.; Kino-Oka, M..; Tone, S; Itchi, T. Production and release of pigments by culture of transformed hairy root of red beet. Journal of Fermentation Bioengineering 1992, 73, 31–36.

    Article  CAS  Google Scholar 

  • Taya, M.; Yakura K.; Kino-Oka, M..; Tone, S; Itchi, T. Influence of medium constituents on enhancement of pigment production by batch culture of red beet hairy roots. Journal of Fermentation Bioengineering 1994, 77, 215–217.

    Article  CAS  Google Scholar 

  • Threfall, D. R.; Whitehead, I. M. Aspects of sesquiterpenoid phytoalexin formation in elicited cell suspension cultures of Nicotiana tabacum -accumulation, metabolism and enzymology. In Manipulating Secondary Metabolism in Culture; Robins, R. J.; Rhodes, M. J. C., Eds.; Cambridge University Press: New York, 1988; pp 41–50.

    Google Scholar 

  • Vincent, K. R.; Scholz, R. G. Separation and quantification of red beet betacyanins and betaxanthins by high pressure liquid chromatography. Journal of Agricultural and Food Chemistry 1978, 28, 540–543.

    Google Scholar 

  • Watson, J. F.; Gabelman,•W. H. Seasonal changes and cultivar differences in pigment concentrations and percent dissolved solids in roots of table beets. Journal of American Horticultural Science 1982, 105, 713–716.

    Google Scholar 

  • Zryd, J. P.; Bauer, J.; Wyler, H.; Lavanchy, P. Pigment biosynthesis and precursor metabolism in red beet semi-continuous cell suspension cultures. In Plant Tissue Culture; Fujiwara, A. Ed.; Maruzen: Tokyo, 1982; pp 387–390.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1999 Springer Science+Business Media New York

About this chapter

Cite this chapter

Mukundan, U., Bhide, V., Dawda, H. (1999). Production of Betalains by Hairy Root Cultures of Beta Vulgaris L. In: Fu, TJ., Singh, G., Curtis, W.R. (eds) Plant Cell and Tissue Culture for the Production of Food Ingredients. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4753-2_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-4753-2_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7155-7

  • Online ISBN: 978-1-4615-4753-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics