Abstract
Objectives
The urinary bladder wall can be separated into two major compartments: the urothelium (mucosa) and the detrusor smooth muscle. Specific dysfunctions of both layers have been linked to ischemia, which may induce significant cellular and subcellular membrane damage via the activation of selective calcium dependent and independent hydrolytic enzymes. Preliminary to investigating changes in cell membrane composition induced by ischemia, we measured the free fatty acid (FFA) and phospholipid (PL) content of normal rabbit bladder muscle and mucosal cellular and subcellular membranes, and characterized the endogenous lipase activity.
Methods
Rabbit bladders were excised and the muscle and mucosal layers separated; each layer was homogenized, then fractionated by differential centrifugation. Endogenous lipase activity of the homogenates, and FFA and PL concentrations of the homogenates and subcellular fractions were measured.
Results
(1) The basal FFA concentration of the mucosal homogenates was 5 times that of the muscle homogenates. (2) The basal PL concentrations of the two tissues were similar. (3) Subcellular studies: FFA concentration was greatest in the mitochondrial fraction of both compartments. In the mucosa, PL concentration was significantly greater in the mitochondria and microsomes than in the other fractions; in the smooth muscle, the PL concentration was highest in the mitochondria. (4) The maximal endogenous lipase activity was 10 times higher in the mucosal homogenates than in the muscle homogenates.
Conclusions
These results are consistent with those of previous studies which indicate that the mucosa is metabolically more active than the resting smooth muscle, which may cause the mucosa to be significantly more sensitive than the muscle to hypoxic/ischemic damage.
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References
Parsons, CL; Greenspan, C; Moore, SW; Mulholland, SG (1977): Role of surface mucin in primary antibacterial defense of bladder. Urology IX, 48–52.
Ruggieri, M., Hanno, P. and Levin, R. M.: Effects of heparin on the adherence of five species of urinary tract pathogens to urinary bladder mucosa. Urological Research, 12: 199–203, 1984.
Levin, R. M., Whitmore, K, Monson, F. C., Witkowski, B. A. and Ruggieri, M.: Trypan blue as an indicator of urothelial integrity, Neurourol Urodyn., 9: 269–280, 1990.
Monson, F. C., Wein, A. J., McKenna, B. A., Whitmore, K. and Levin, R. M.: Indigocarmine as a quantitative indicator of urothelial integrity. J. Urol., 145: 842–845, 1991.
Parsons, CL; Boychuk, D; Jones, S; Hurst, R; Callahan, H (1990): Bladder surface glycosaminoglycans: an epithelial permeability barrier. J. Urol. 143, 139–142.
Lilly, JD; Parsons, CL (1990): Bladder surface glycosaminoglycans is a human epithelial permeability barrier. Surg. Gynecol. Obstet. 171, 493–496.
Parsons, CL; Lilly, JD; Stein, P (1991): Epithelial dysfunction in nonbacterial cystitis (interstitial cystitis). J. Urol. 145, 732–735.
Steers, W.D. Physiology of the urinary bladder. In Cambells Urology, (eds. Walsh, P.C., Retik, A.B., Stamey, T.A., and Vaughan, E.D. Jr.) Saunders, Phila., 1992, pp 142–176.
Wein AJ, Levin RM, Barrett DM: Voiding function: Relevant anatomy, physiology, and pharmacology. In Gillenwater JY, Grayhack JT, Howards SS, Duckett JD (eds): Adult and Pediatric Urology, 2nd ed. Chicago, Year Book Medical Publishers, pp. 933–999, 1991.
Anderson, K.E. Pharmacology of lower urinary tract smooth muscles and penile erectile tissue. Pharmacol. Rev. 45: 253–307, 1993.
Grayhack, J.T. and Kozlowski, J.M. Benign Prostatic Hyperplasia. In Adult and Pediatric Urology (Gillenwater, J.Y., Grayhack, J.T., Howards, S.S., and Duckett, J.W. eds.) Year Book Med. Pub. Inc. (Chicago), pp 1062–1126, 1987.
Sterling, A.M., Ritter, R.C., and Zinner, N.R. The physical basis of obstructive uropathy, in Benign Prostatic Hypertrophy, Springer-Verlag, N.Y. 1983 (pp 433–442)
Levin, R. M., Longhurst, P. A., Monson, F. C., Haugaard, N., and Wein, A. J.: Experimental studies on bladder outlet obstruction. In: Prostate Diseases. Edited by H. Lepor, Lepor MD, R.K. Lawson, Lawson MD. Philadelphia: W.B. Saunders Co., pp. 119–130, 1993.
Levin, R. M., Longhurst, P. A., Monson, F. C., Kato, K., and Wein, A. J.: Effect of bladder outlet obstruction on the morphology, physiology, and pharmacology of the bladder. Prostate, Supp. 3: 9–26, 1990.
Levin, R. M., Levin, S. S. and Wein, A. J.: Etiology of Incontinence: A review and hypothesis. Scandanavian J. Urol., Nephrol. 30, Supp. 179: 15–25, 1996.
Levin, R.M., Levin, S.S., Zhao, Y., and Buttyan, R. Cellular and molecular aspects of bladder hypertrophy. Eur. Urol. 32: (supp) 15–21, 1997.
Greenland, JE; Hvistendahl, JJ; Andersen, H; Jorgensen, TM; Constantinou, CE; McMurray, G; Brading, AF; Frokiaer, J (1997): Detrusor and kidney blood flow is reduced in response to early bladder outlet obstruction in pigs. J. Urol. 157(4, Suppl), 172.
Ruggieri, M. R., Hanno, P. M., Samadzdeh, S., Johnson, E. W. and Levin, R. M.: Heparin inhibition of increased bacterial adherence following overdistention, ischemia, and partial outlet obstruction of the rabbit urinary bladder. J. Urology, 136: 132–135, 1986.
Lin, AT; Chen, MT; Yang, CH; Chang, LS (1995): Blood flow of the urinary bladder: effects of outlet obstruction and correlation with bioenergetic metabolism. Neurourol. Urodynam. 14, 285–292.
Brading, A.F. Alterations in the physiological properties of urinary bladder smooth muscle caused by bladder emtpying against an obstruction. Scand. J. Urol. Nephrol. 184: 512–58, 1997.
Hypolite, J. A., Longhurst, P. A., Gong, C., Briscoe, J., Wein, A. J. and Levin, R. M.: Metabolic studies on rabbit bladder smooth muscle and mucosal epithelium. Mol. Cell. Biochem., 125: 35–42, 1993
Levin, R. M., Hypolite, J. A., Haugaard, N. and Wein, A. J.: Comparative response of rabbit bladder smooth muscle and mucosa to anoxia. Neurorurol. Urodyn., 15: 79–84, 1996.
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O’Connor, L.J., Nicholas, T., Levin, R.M. (1999). Subcellular Distribution of Free Fatty Acids, Phospholipids, and Endogenous Lipase Activity of Rabbit Urinary Bladder Smooth Muscle and Mucosa. In: Baskin, L.S., Hayward, S.W. (eds) Advances in Bladder Research. Advances in Experimental Medicine and Biology, vol 462. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4737-2_20
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DOI: https://doi.org/10.1007/978-1-4615-4737-2_20
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