Advertisement

Manipulation of Plant Oil Composition for the Production of Valuable Chemicals

Progress, Problems, and Prospects
  • Denis J. Murphy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 464)

Abstract

The first transgenic crop with a modified seed oil composition was released for commercial use in 1995. Many additional transgenic rapeseed varieties expressing novel seed oils or proteins are now under development. Recent research advances in Arabidopsis molecular genetics and the emerging relatedness between the Arabidopsis and Brassica genomes will enable more radical manipulation of many key agronomic traits in rapeseed. These will range from greatly improved seed oil yield to the production of seed oils with defined fatty acid contents ranging from C8 to C24 chain lengths and including many desirable functionalities including double bonds, triple bonds, hydroxy and epoxide groups. Much of the technology currently being development in rapeseed and soybean oil modification will be directly applicable to other oil crops including high yield tropical perennials such as oil palm.

Keywords

Lauric Acid Fatty Acid Desaturases Desaturase Gene Valuable Chemical Fatty Acid Chain Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Arondel, V.; Lemieux, B.; Hwang, I.; Gibson, S.; Goodman, H. M.; Somerville, C. R. Map-based cloning of a gene controlling Omega-3 fatty acid desaturation in Arabidopsis. Science 1992, 258, 1353–1355.PubMedCrossRefGoogle Scholar
  2. Beremand, P. D.; Nunberg, A. N.; Reddy, A. S.; Thomas, T. L. Production of m-linolenic acid by transgenic plants expressing cyanobacterial or plant Æ-desaturase genes. In Physiology, Biochemistry and Molecular Biology of Plant Lipids; Williams J. P., Khan M. U, Lern N. W., Eds.; Kluwer: Dordrecht, 1997; pp 351–353.Google Scholar
  3. Block, G.; Patterson, B.; Suber, A. Fruit, vegetables, and cancer prevention — a review of the epidemiologic evidence. Nutrition and Cancer 1992,18, 1–19.PubMedCrossRefGoogle Scholar
  4. Cahoon, E. B.; Shanklin, J. Approaches to the design of acyl-ACP desaturases with altered fatty acid chain-length and double bond positional specificities. In Physiology, Biochemistry and Molecular Biology of Plant Lip-ids; Williams J. P., Khan M. U, Lern N. W., Eds.; Kluwer: Dordrecht, 1997; pp 374–376.Google Scholar
  5. Carruthers, S. P.; Marsh, J. S.; Turner, P. W.; Ellis, F.; Murphy, D. J.; Slabas, T.; Chapman, B. A. Industrial markers for UK produced oilseeds. Centre for Agricultural Strategy. Research Review No 0S9. University of Reading; Centre for Agricultural Strategy: University of Reading, 1995.Google Scholar
  6. Cheah, S. C. 22nd World Congress & Exhibition of the International Society for Fat Research, 8–12 Sept, 1997, Kuala Lumpur, ISF, Champaign, Illinois, 1997.Google Scholar
  7. Cohen, Z.; Shiran, D.; Khozine, I.; Heimer, Y. M. Fatty acid unsaturation in the red alga Porphyridium cruentum. Is the méthylene interrupted nature of polyunsaturated fatty acids an intrinsic property of the desaturases? Biochim Biophys Acta 1997, 1344, 59–64.PubMedCrossRefGoogle Scholar
  8. Corley, R. H. V.; Lee, C. H.; Law, I. W.; Wong, C. Y. Abnormal flower development in oil palm clones. Planter 1986, 62, 233–240.Google Scholar
  9. Dehesh, K. Molecular and biochemical analysis of medium-chain lipid biosynthesis. Abstract 100. 5th Int. Congr. of Plant Mol Biol, 21–27 September 1997; Kluwer, Dordrecht, 1997.Google Scholar
  10. Kinney, A. J. Genetic engineering of fatty acid metabolism in soybeans. Abstract 99. 5th Int. Congr. of Plant Mol. Biol., 21–27 September 1997; Kluwer, Dordrecht, 1997.Google Scholar
  11. Knutzon, D. S.; Thompson, G. A.; Radke, S. E.; Johnson, W. B.; Knauf, V. C.; Kridl, J. C. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc Natl Acad Sci USA 1992, 89, 2624–2628.PubMedCrossRefGoogle Scholar
  12. Lindqvist, Y.; Huang, W.; Schneider, G.; Shanklin, J. Crystal structure of a delta-9 stearoyl-acyl carrier protein de-saturase from castor seed and its relationship to other di-iron proteins. EMBO Journal 1996, 15, 4081–4092.PubMedGoogle Scholar
  13. Moloney, M. M.; Walker, J.; Sharma, K. High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep. 1989, 8, 238–242.CrossRefGoogle Scholar
  14. Murphy, D. J.; Piffanelli, P. Fatty acid desaturases: structure mechanism and regulation. In Plant Lipid Biosynthesis: Recent Advances of Agricultural Importance; Harwood, J. L., Ed.; Cambridge University Press, 1998, pp. 95–130.Google Scholar
  15. Murphy, D. J. Genomics: Commercial Opportunities from a Scientific Revolution, University of Cambridge; Society of Chemical Industry, 1998, pp. 199–210.Google Scholar
  16. Murphy, D. J. Biotechnological improvement of oil crops. TIBTECH 1996,14, 206–213.CrossRefGoogle Scholar
  17. Okuley, J.; Lightner, J.; Feldmann, K.; Yadav, N.; Lark, E.; Browse, J. Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 1994, 6, 147–158.PubMedGoogle Scholar
  18. Parkin, I. A.; Cavell, A.; Oldknow, J.; Lydiate, D. J.; Trick, M. AGenome synteny between Arabidopsis thaliana and Brassica napus.,@ John Innes Centre Annual Report, 1997.Google Scholar
  19. Parmenter, D. L.; Boothe, J. G.; van Rooijen, G. J. H.; Yeung, E. C.; Moloney, M. M. Production of biologically active hirudin in plant seeds using oleosin partitioning. Plant Mol.Biol. 1995,29, 1167–1180.PubMedCrossRefGoogle Scholar
  20. Rafferty, J. B.; Simon, J. W.; Baldock, C.; Artymiuk, R J.; Baker, R J.; Stuitje, A. R.; Slabas, A. R.; Rice, D. W. Common themes in redox chemistry emerge from the x-ray structure of oilseed rape (Brassica napus) enoyt acyl carrier protein reductase. Struct. 1995, 3, 927–938.CrossRefGoogle Scholar
  21. Sadanandom, A.; Piffanelli, P.; Knott, T.; Sharpe, A.; Lydiate, D.; Murphy, D. J.; Fairbairn, D. Identification of a peptide methionine sulfoxide reductase gene in an oleosin promotor from Brassica napus. Plant Journal 1996,10, 235–242.PubMedCrossRefGoogle Scholar
  22. Shanklin, J.; Cahoon, E. B.; Whittle, E.; Lindqvist, Y.; Huang, W.; Schneider, G.; Schmidt, H. Structure-function studies on desaturases and related hydrocarbon hydroxylases. In Physiology, Biochemistry and Molecular Biology of Plant Lipids; Williams J. P., Khan M. U, Lern N. W, Eds.; Kluwer: Dordrecht, 1997; pp 6–10.Google Scholar
  23. Shiran, D.; Khozin, I.; Heimer, Y. M. Biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum. I: The use of externally supplied fatty acids. Lipids 1996,31, 1277–1282.PubMedCrossRefGoogle Scholar
  24. Somerville, C. The physical map of an Arabidopsis chromosome. Trends in Plant Sci 1996,1, 2.CrossRefGoogle Scholar
  25. Tawfiq, N.; Heaney, R.; Plumb, J.; Fenwick, G.; Musk, S.; Williamson, G. Dietary glucosinolates as blocking agents against carcinogenesis: glucosinolate breakdown products assessed by induction of quinone reductase activity in murine hepalclc7 cells. Carcinogenesis. 1995,16, 1191–1194.PubMedCrossRefGoogle Scholar
  26. Thompson, G. A.; Li, C. Altered fatty acid composition of membrane lipid in seeds and seedling tissues of high-saturate canolas. In Physiology, Biochemistry and Molecular Biology of Plant Lipids; Williams J. P., Khan M. U, Lem N. W, Eds.; Kluwer: Dordrecht, 1997; pp 313–315.Google Scholar
  27. van de Loo, F. J.; Broun, P.; Turner, S.; Somerville, C. An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proc Natl Acad Sci USA 1995, 92, 6743–6747.PubMedCrossRefGoogle Scholar
  28. Voelker, T. A.; Hayes, T. R.; Cranmer, A. M.; Turner, J. C.; Davies, H. M. Genetic engineering of a quantitative trait: metabolic and genetic parameters influencing the accumulation of laurate in rapeseed. Plant J. 1996, 9, 229–241.CrossRefGoogle Scholar
  29. Yadav, N. S.; Wierzbicki, A.; Aegerter, M.; Caster, C. S.; Perez-Grau, L.; Kinney, A. J.; Hitz, W. D.; Booth, J. R. J.; Schweiger, B.; Stecca, K. L.; Allen, S. M.; Blackweil, M.; Reiter, R. S.; Carlson, T. J.; Russell, S. H.; Feldmann, K. A.; Pierce, J.; Browse, J. Cloning of higher plant U-3 fatty acid desaturases. Plant Physiol 1993,103,467–476.PubMedCrossRefGoogle Scholar
  30. Zhang, Y.; Kensler, T. W.; Cho, C. G.; Posner, G. H.; Talalay, P. Proc Natl Acad Sci USA 1994 91, 3147–3150.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Denis J. Murphy
    • 1
  1. 1.Department of Brassica and Oilseeds ResearchJohn Innes CentreNorwichUK

Personalised recommendations