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Molecular Strategies to Improve The Nutritional Quality of Legume Proteins

  • Benito O. de Lumen
  • Alfredo F. Galvez
  • M. Jamela Revilleza
  • Deanne C. Krenz
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 464)

Abstract

Legume proteins are relatively deficient in the sulfur amino acids. Among the different strategies to increase the sulfur amino acid contents of legumes, we have chosen to increase the biosynthesis of endogenous, non-abundant sulfur-rich proteins we have identified in soybean seed. We identified and isolated an 8 kDa sulfur-rich protein from the albumin fraction of soybean seed. Subsequently, a cDNA designated Gm2S-l, has been isolated and characterized. The Gm2S-l cDNA encodes a pre-proprotein that contains a signal peptide and a precursor protein that undergoes post-translational processing to yield the mature 8 kDa protein containing 7.8% methionine and 7.8% cysteine. The Gm2S-l gene is an ideal candidate for overexpression to improve the nutritional quality of soybean and other legumes.

Keywords

Essential Amino Acid Nutritional Quality Soybean Seed Seed Storage Protein Methionine Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Benito O. de Lumen
    • 1
  • Alfredo F. Galvez
    • 1
  • M. Jamela Revilleza
    • 2
  • Deanne C. Krenz
    • 1
  1. 1.Division of Nutritional Sciences and ToxicologyUniversity of CaliforniaBerkeleyUSA
  2. 2.Institute of ChemistryUniversity of the Philippines at Los Banos College LagunaPhilippines

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