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Determination of N1-Methylnicotinamide by Hplc Postcolumn Photoirradiation

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Tryptophan, Serotonin, and Melatonin

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 467))

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Abstract

A method for the determination of N’-Methylnicotinamide (NMNA) was developed by the fluorescence formed by UV irradiation after separation by HPLC with a mobile phase containing hydrogen peroxide and 18-crown-6. This method was applied to human urine and serum.

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References

  • Mawatari, K., Andoh, Y., Nakamaru, Y., Nishiyama, K., Iinuma, F., and Watanabe, M., 1998, Fluorometric determination of N1-methylnicotinamide in human urine and serum by liquid chromatography postcolumn photoirradiation with a mobile phase containing 18-crown-6, Anal. chim. acta, 363:133–140.

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  • Mawatari, K., Iinuma, F., and Watanabe, M., 1991, Determination of nicotinic acid and nicotinamide in human serum by high performance liquid chromatography with postcolumn ultraviolet-irradiation and fluorescence detection, Anal. Sci., 7:733.

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© 1999 Springer Science+Business Media New York

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Mawatari, Ki., Iinuma, F., Watanabe, M. (1999). Determination of N1-Methylnicotinamide by Hplc Postcolumn Photoirradiation. In: Huether, G., Kochen, W., Simat, T.J., Steinhart, H. (eds) Tryptophan, Serotonin, and Melatonin. Advances in Experimental Medicine and Biology, vol 467. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4709-9_103

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  • DOI: https://doi.org/10.1007/978-1-4615-4709-9_103

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7133-5

  • Online ISBN: 978-1-4615-4709-9

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