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Direct Isolation of Specific Chromosomal Regions and Entire Genes by TAR Cloning

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Genetic Engineering

Part of the book series: Genetic Engineering ((GEPM,volume 21))

Abstract

Human genome analysis has been advanced considerably by the development of Yeast Artificial Chromosome (YAC) cloning systems (1). YAC techniques have made possible the isolation of megabase DNA fragments, thereby greatly simplifying the physical mapping of chromosomes and the cloning of entire genes. Overlapping YACs can be conveniently modified by homologous recombination in vivo to construct a single YAC that contains a large genomic locus (2), or to create a new YAC with alterations in specific regions (3). This approach can be used to generate YACs which, upon transfer into mammalian cells, will contribute to the functional analysis of the isolated region.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, 27709, USA

    Vladimir Larionov

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  1. Vladimir Larionov
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Editors and Affiliations

  1. Brookhaven National Laboratory, Upton, New York

    Jane K. Setlow

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© 1999 Springer Science+Business Media New York

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Larionov, V. (1999). Direct Isolation of Specific Chromosomal Regions and Entire Genes by TAR Cloning. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 21. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4707-5_3

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  • DOI: https://doi.org/10.1007/978-1-4615-4707-5_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-7132-8

  • Online ISBN: 978-1-4615-4707-5

  • eBook Packages: Springer Book Archive

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