Summary
Mitochondrial gene expression and enzyme activities were analyzed in exercise-induced hypertrophic rat heart and SHR heart muscles. Exercise-induced hypertrophic hearts (7% increase in weight relative to body weight compared to that of sedentary rats) were prepared by endurance training of rats for 12 weeks (90min/day, 5 days/week). Although the activity of cytochrome oxidase and concentrations of cytochrome b mRNA and mitochondrial DNA (mtDNA) in gastrocnemius muscle were increased approximately 1.5-fold by training, those in the heart were not altered, suggesting that adaptation in the heart to endurance training is attained by increasing the muscle mass, not by mitochondrial gene expression. The concentration of mtDNA in the SHR heart, which increased in relative weight by 62% compared to that of normal rats, was not altered by hypertrophy, but the concentration of cytochrome b mRNA and activities of citrate synthase and cytochrome oxidase in the heart tended to be decreased by hypertrophy. These results suggest that the adaptive response of mitochondrial gene expression to cardiac hypertrophy is different in exercise- and hypertension-induced hypertrophy.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson SA, Bankier T, Barrell BG, de Bruijn MHL, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG. 1981. Sequence and organization of the human mitochondrial genome. Nature 290:457–465.
Tzagoloff A, Myers AM. 1986. Genetics of mitochondrial biogenesis. Annu Rev Biochem 55:249–285.
Clayton DA. 1991. Replication and transcription of vertebrate mitochondrial DNA. Annu Rev Cell Biol 7:453–479.
Murakami T, Shimomura Y, Fujitsuka N, Nakai N, Sugiyama S, Ozawa T, Sokabe M, Horai S, Toduyama K, Suzuki M. 1994. Enzymatic and genetic adaptation of soleus muscle mitochondria to physical training in rats. Am J Physiol 267:E388–E395.
Holloszy JO, Booth FW. 1976. Biochemical adaptations to endurance exercise in muscle. Annu Rev Physiol 38:273–291.
Rabinowits M, Zak R. 1975. Mitochondria and cardiac hypertrophy. Circ Res 36:367–376.
Oscai LB, Molé PA, Brei B, Holloszy JO. 1971. Cardiac growth and respiratory enzyme levels in male rats subjected to a running program. Am J Physiol 220:1238–1241.
Oscai LB, Molé PA, Holloszy JO. 1971. Effects of exercise on cardiac weight and mitochondria in male and female rats. Am J Physiol 220:1944–1948.
Hickson RC, Hammons GT, Holoszy JO. 1979. Development and regression of exercise-induced cardiac hypertrophy in rats. Am J Physiol 236:H268–H272.
Murakami T, Shimomura Y, Fujitsuka N, Sugiyama S. 1995. Different adaptation to endurance training between heart and gastrocnemius muscle mitochondria in rats. Biochem Mol Biol Int 36:285–290.
Tokoro T, Ito H, Maenishi O, Suzuki T. 1995. Mitochondrial abnormalities in hypertrophied myocardium of stroke-prone spontaneously hypertensive rats. Clin Exp Pharmacol Physiol Suppl 1:S268–S269.
Tokoro T, Ito H, Suzuki T. 1996. Alterations in mitochondrial DNA and enzyme activities in hypertrophied myocardium of stroke-prone SHRs. Clin Exp Hypertens 18:595–606.
Chen L, Tian X, Song L. 1995. Biochemical and biophysical characteristics of mitochondria in the hypertrophic hearts from hypertensive rats. Clin Med J (Engl) 108:361–366.
Bunag RD. 1973. Validation in awake rats of tail-cuff method for measuring systolic pressure. J Appl Physiol 34:279–282.
Smith L. 1955. Spectrophotometric assay of cytochrome c oxidase. Methods Biochem Anal 2:427–434.
Srere PA. 1969. Citrate synthase. Methods Enzymol 13:3–5.
Labarca C, Paigen K. 1980. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem 102:344–352.
Ott RL. 1993. An Introduction to Statistical Methods and Data Analysis (4th ed.). Belmont, CA: Wadsworth.
Morrison PR, Biggs RB, Booth FW. 1989. Daily running for 2wk and mRNAs for cytochrome c and α-actin in rat skeletal muscle. Am J Physiol 257:C936–C939.
Annex BH, Kraus WE, Dohm GL, Williams RS. 1991. Mitochondrial biogenesis in striated muscles: rapid induction of citrate synthase mRNA by nerve stimulation. Am J Physiol 260:C266–C270.
Williams RS. 1986. Mitochondrial gene expression in mammalian striated muscle. Evidence that variation in gene dosage is the major regulatory event. J Biol Chem 261:12390–12394.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Murakami, T., Shimomura, Y., Zhihao, L., Shimizu, K., Sugiyama, S. (2000). Mitochondrial Gene Expression in Hypertrophic Cardiac Muscles in Rats. In: Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Hypertrophied Heart. Progress in Experimental Cardiology, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4423-4_4
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4423-4_4
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6991-2
Online ISBN: 978-1-4615-4423-4
eBook Packages: Springer Book Archive