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The Hypertrophied Heart pp 197–206Cite as

Stimulation of Mitogen-Activated Protein Kinases ERK-1 and ERK-2 by H2O2 in Vascular Smooth Muscle Cells

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Part of the book series: Progress in Experimental Cardiology ((PREC,volume 3))

Summary

The possible roles of the mitogen-activated protein kinases (MAPKs) ERK-1 and ERK-2 in mediating growth-promoting and hypertrophic responses were investigated by examining the effect of H2O2 on ERK-1 and ERK-2 phosphorylation and activation in an established vascular smooth muscle cell (VSMC) line (A10). H2O2 treatment of VSMCs stimulated the phosphorylation of both ERK-1 and ERK-2 in a concentration-dependent manner with maximal impact at the dose level of 2mM H2O2. Treatment of cells with aminotriazole, an inhibitor of catalase, potentiated the effect of H2O2 and reduced the H2O2 concentration required to elicit the maximum response. In addition, PD98059, an inhibitor of MAPK kinase (MEK), and wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), attenuated the H2O2-induced activation of ERK-1 and ERK-2. H2O2 was also found to stimulate PI3K activity, which was inhibited by wortmannin. Taken together, these data demonstrate that oxidative stress-induced ERK activation is mediated, at least in part, through a PI3K-dependent pathway and suggest a role of this pathway in eliciting growth-promoting and hypertrophic responses to H2O2 in VSMCs.

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Author information

Authors and Affiliations

  1. Department of Medicine, University of Montreal Research Center-Centre hospitalier de l’Université de Montréal (CHUM), Campus Hôtel-Dieu, 3840 St. Urbain Street, Montreal, Quebec, H2W 1T8, Canada

    Ashok K. Srivastava & Sanjay K. Pandey

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  1. Ashok K. Srivastava
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  2. Sanjay K. Pandey
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine, Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda M.D., Ph.D. (Associate Professor) (Associate Professor)

  2. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor Emeritus) (Professor Emeritus)

  3. Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon) (Distinguished Professor and Director) (Distinguished Professor and Director)

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Srivastava, A.K., Pandey, S.K. (2000). Stimulation of Mitogen-Activated Protein Kinases ERK-1 and ERK-2 by H2O2 in Vascular Smooth Muscle Cells. In: Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Hypertrophied Heart. Progress in Experimental Cardiology, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4423-4_16

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  • DOI: https://doi.org/10.1007/978-1-4615-4423-4_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6991-2

  • Online ISBN: 978-1-4615-4423-4

  • eBook Packages: Springer Book Archive

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