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Methods in Mammary Gland Biology and Breast Cancer Research pp 37–50Cite as

Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease

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Abstract

Immune deficient mice may be used to study the development, growth, and metastasis of human breast cancer. Progression of the MCF10 premalignant human breast epithelial cell lines occurs in xenografts, providing a model to study early events in human breast disease. Tumorigenicity of breast cancer cell lines may be enhanced either by suspending cells in Matrigel prior to injection into the subcutis or by injecting the cells into a mammary fat pad. Metastasis is enhanced by implanting breast cancer xenografts into mammary fat pads.

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Abbreviations

AH:

atypical hyperplasia

DCIS:

ductal carcinoma in situ

SCID:

severe combined immune deficiency

Xid:

x-linked immune deficiency

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Author information

Authors and Affiliations

  1. Karmanos Cancer Institute, and School of Medicine, Wayne State University, Detroit, Michigan, 48201, USA

    Fred R. Miller & Gloria H. Heppner

Authors
  1. Fred R. Miller
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  2. Gloria H. Heppner
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Editor information

Editors and Affiliations

  1. Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, New York, 14263, USA

    Margot M. Ip

  2. Roswell Park Cancer Research Institute, Buffalo, New York, USA

    Bonnie B. Asch

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© 2000 Springer Science+Business Media New York

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Miller, F.R., Heppner, G.H. (2000). Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_4

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  • DOI: https://doi.org/10.1007/978-1-4615-4295-7_4

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6927-1

  • Online ISBN: 978-1-4615-4295-7

  • eBook Packages: Springer Book Archive

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