Abstract
Over the past decade the tools of gene targeting have permitted an unparalleled insight into genetic pathways that control mammary development and tumorigenesis in the mouse. However, the role of many genes in development and disease remains elusive, since their deletion from the mouse genome is either lethal for the mouse or does not mimic human disease progression. Thus, targeting gene deletions or modifications precisely to mammary epithelial cells during distinct time windows is a promising approach to establish high-fidelity mouse models for the study of development and disease.
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Abbreviations
- Ad:
-
adenoviral
- BLG:
-
β-lactoglobulin
- CMV:
-
cytomegalovirus
- ES:
-
embryonic stem
- GOI:
-
gene of interest
- LTR:
-
long terminal repeat
- MMTV:
-
mouse mammary tumor virus
- WAP:
-
whey acidic protein
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Wagner, KU., Rucker, E.B., Hennighausen, L. (2000). Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissue. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_24
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_24
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