Abstract
Insertion of specific exogenous genes into the mammary parenchyma genome has been shown to have many important uses, including production of experimental models and production of commercially valuable proteins in milk. Most transgenic mammary tissue has thus far been produced by germ-line gene transfer. Here we describe a rapid and inexpensive alternative method in which genes of interest are placed directly into the in situ mammary ductal epithelium using a “gene-therapy-like” approach. The gene of interest is cloned into a replication-incompetent retroviral vector. The vector is produced to yield very high titers and is then infused into the central mammary duct of mammals ranging from rats to goats. Larger mammals are used mainly for protein production, and rats are used to develop models for the study of mammary biology and carcinogenesis.
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Abbreviations
- MMTV:
-
mouse mammary tumor virus
- LTR:
-
long terminal repeat
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Thompson, T.A., Gould, M.N. (2000). Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectors. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_22
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_22
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