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mRNA In Situ Hybridization in the Mammary Gland

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Methods in Mammary Gland Biology and Breast Cancer Research

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Abstract

The localization of mRNA expression in the mammary gland is technically challenging due to the unique structural problems posed by breast tissues. These include closely interdigitated and sparse layers of tissue, high levels of extracellular matrix, and large quantities of adipose tissue. This chapter describes an in situ hybridization method using radiolabeled oligonucleotides that was developed to allow the detection of the rarest of messenger RNAs in many types of breast sample.

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Abbreviations

(BCIP/NBT):

bromochloroindolyl phosphate/nitro blue tetrazolium

(DEPC):

diethyl pyrocarbonate

(DIG):

digoxigenin

(EST):

expressed sequence tag

(ISH):

in situ hybridization

(PFA):

paraformaldehyde

(PBS):

phosphate buffered saline

(TGF):

transforming growth factor

(SSC):

0.15 M sodium chloride/sodium citrate pH 7.0

References

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Author information

Authors and Affiliations

  1. Institute of Cancer Research, The Breakthrough Toby Robins Breast Cancer Centre, London, SW3 6JB, England

    Steven Weber-Hall & Trevor Dale

Authors
  1. Steven Weber-Hall
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  2. Trevor Dale
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Editor information

Editors and Affiliations

  1. Department of Pharmacology and Therapeutics, Roswell Park Cancer Institute, Buffalo, New York, 14263, USA

    Margot M. Ip

  2. Roswell Park Cancer Research Institute, Buffalo, New York, USA

    Bonnie B. Asch

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© 2000 Springer Science+Business Media New York

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Weber-Hall, S., Dale, T. (2000). mRNA In Situ Hybridization in the Mammary Gland. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_19

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  • DOI: https://doi.org/10.1007/978-1-4615-4295-7_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6927-1

  • Online ISBN: 978-1-4615-4295-7

  • eBook Packages: Springer Book Archive

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