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Molecular Tools for the Study of Titin’s Differential Expression

  • Thomas Centner
  • Francoise Fougerousse
  • Alexandra Freiburg
  • Christian Witt
  • Jacque S. Beckmann
  • Henk Granzier
  • Karoly Trombitás
  • Carol C. Gregorio
  • Siegfried Labeit
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 481)

Abstract

Although vertebrate genomes appear to contain only one titin gene, a large variety of quite distinct titin isoforms are expressed in striated muscle tissues. The isoforms appear to be generated by a series of complex, not yet fully characterized differential splicing mechanisms. Here, we provide an overview of the titin-specific antibodies that have been raised by our laboratory to study individual differentially expressed isoforms of titin. The staining patterns obtained in different tissues will contribute to the identification of both the particular titin isoforms that are expressed in the different tissues, as well as their intracellular distributions. In addition, antibodies to titin that are available are rapidly allowing for the refinement of our knowledge of titin’s elastic spring properties. Knowledge of the nature and structure of vertebrate titins that may also be expressed in nonmuscle tissues may be broadened using these antibodies.

Keywords

Thin Filament Immunoelectron Microscopy Study Titin Isoforms Cardiac Titin PEVK Segment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Thomas Centner
    • 1
  • Francoise Fougerousse
    • 2
  • Alexandra Freiburg
    • 3
  • Christian Witt
    • 1
  • Jacque S. Beckmann
    • 2
  • Henk Granzier
    • 4
  • Karoly Trombitás
    • 4
  • Carol C. Gregorio
    • 5
  • Siegfried Labeit
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany
  2. 2.Genethon-URA1922EvryFrance
  3. 3.Institut für Anästhesiologie und Operative IntensivmedizinUniversitätsklinikum MannheimMannheimGermany
  4. 4.Department of Veterinary and Comparative Anatomy, Pharmacology and PhysiologyWashington State UniversityPullmanUSA
  5. 5.Departments of Cell Biology and Anatomy, and Molecular and Cellular BiologyUniversity of ArizonaTucsonUSA

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