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Role of the Elastic Protein Projectin in Stretch Activation and Work Output of Drosophila Flight Muscles

  • Jim O. Vigoreaux
  • Jeffrey R. Moore
  • David W. Maughan
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 481)

Abstract

We examine how the stretch activation response of the Drosophila indirect flight muscles (IFM) is affected by the projectin mutation bent Dominant . IFM from flies heterozygous for this mutation (bent D / +) produce ~85% full length projectin and ~15% truncated projectin lacking the kinase domain and more C-terminal sequences. Passive stiffness and power output of mutant fibers is similar to that of wild-type (+/+) fibers, but the amplitude of the stretch activation response (delayed tension rise) was significantly reduced. Measurement of actomyosin kinetics by sinusoidal analysis revealed that the apparent rate constant of the delayed tension rise (2πb) increased in proportion to the decrease in amplitude, accounting for the near wild-type levels of power output and nearly normal flight ability. These results suggest that projectin plays a crucial role in stretch activation, possibly through its protein kinase activity, by modulating crossbridge recruitment and kinetics

Keywords

Kinase Domain Myosin Light Chain Kinase Apparent Rate Constant Flight Muscle Regulatory Light Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Jim O. Vigoreaux
    • 1
    • 3
  • Jeffrey R. Moore
    • 2
    • 3
  • David W. Maughan
    • 2
    • 3
  1. 1.Department of BiologyThe University of VermontBurlingtonUSA
  2. 2.Department of Molecular Physiology and BiophysicsThe University of VermontBurlingtonUSA
  3. 3.Cell and Molecular Biology ProgramThe University of VermontBurlingtonUSA

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