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Titin as a Chromosomal Protein

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Book cover Elastic Filaments of the Cell

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 481))

Abstract

We identified titin as a chromosomal protein using a human autoimmune scleroderma serum. We cloned the corresponding gene in the fruitfly, Droso-phila melanogaster. We have demonstrated that titin is not only expressed and localized in striated muscle but is also distributed uniformly on condensed mitotic chromosomes using multiple antibodies directed against different domains of both Drosophila and vertebrate titin. Titin is a giant sarcomeric protein responsible for the elasticity of striated muscle. Titin may also function as a molecular scaffold during myofibril assembly. We hypothesize that titin is a component of chromosomes that may function to determine chromosome structure and provide elasticity, playing a role similar to that proposed for titin in muscle. We have identified mutations in Drosophila Titin (D-Titin) and are characterizing phenotypes in muscle and chromosomes.

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Authors and Affiliations

  1. Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Cristina Machado & Deborah J. Andrew

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  1. Cristina Machado
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  2. Deborah J. Andrew
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Editors and Affiliations

  1. Washington State University, Pullman, Washington, USA

    Henk L. Granzier

  2. University of Washington, Seattle, Washington, USA

    Gerald H. Pollack

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Machado, C., Andrew, D.J. (2000). Titin as a Chromosomal Protein. In: Granzier, H.L., Pollack, G.H. (eds) Elastic Filaments of the Cell. Advances in Experimental Medicine and Biology, vol 481. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4267-4_13

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  • DOI: https://doi.org/10.1007/978-1-4615-4267-4_13

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