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Angiogenesis pp 7–34Cite as

Examining New Models for the Study of Autocrine and Paracrine Mechanisms of Angiogenesis Through FGF2-Transfected Endothelial and Tumour Cells

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 476))

Abstract

Angiogenesis is the process of generating new capillary blood vessels. Uncontrolled endothelial cell proliferation is observed in tumour neovascularization. Several growth factors and cytokines have been shown to stimulate endothelial cell proliferation in vitroand in vivoand among them FGF2 was one of the first to be characterised. FGF2 is a Mr18,000 heparinbinding cationic polypeptide that induces proliferation, migration, and protease production in endothelial cells in culture and neovascularization in vivo.FGF2 interacts with endothelial cells through two distinct classes of receptors, the high affinity tyrosine-kinase receptors (FGFRs) and low affinity heparan sulfate proteoglycans (HSPGs) present on the cell surface and in the extracellular matrix. Besides experimental evidence for paracrine mode of action for FGF2, some observations raise the hypothesis that FGF2 may also play an autocrine role in endothelial cells. FGF2 may therefore represent a target for antiangiogenic therapies. In order to assess the angiostatic potential of different classes of compounds, novel experimental models have been developed based on the autocrine and/or the paracrine capacity of FGF2.

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Authors and Affiliations

  1. Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, 25123, Brescia, Italy

    Marco Presta, Marco Rusnati, Patrizia Dell’Era, Elena Tanghetti, Chiara Urbinati, Roberta Giuliani & Daria Leali

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  1. Marco Presta
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  2. Marco Rusnati
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  3. Patrizia Dell’Era
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  4. Elena Tanghetti
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  6. Roberta Giuliani
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  1. University of Patras Medical School, Patras, Greece

    Michael E. Maragoudakis

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Presta, M. et al. (2000). Examining New Models for the Study of Autocrine and Paracrine Mechanisms of Angiogenesis Through FGF2-Transfected Endothelial and Tumour Cells. In: Maragoudakis, M.E. (eds) Angiogenesis. Advances in Experimental Medicine and Biology, vol 476. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4221-6_2

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