Abstract
Bradykinin (BK) contributes to the inflammatory response inducing vasodilation of postcapillary venules and has been demonstrated to induce neovascular growth in subcutaneous rat sponges. In this study the ability of BK to stimulate cell growth and migration in cultured endothelium from coronary postcapillary venules (CVEC) has been investigated. BK promotes growth of endothelial cells and its mitogenic activity involves c-Fos expression. Only the Bl receptor appears to be responsible for the proliferation induced by BK and suggests that this type of receptor might be implicated in favouring angiogenesis of coronary venules. Phospholipase C (PLC) activation and the endogenous upregulation of nitric oxide synthase (NOS) were monitored in response to Bl and B2 receptor activation. BK-induced IP turnover was triggered by B2 activation, while NOS activation was linked to the Bl receptor. NOS inhibition in CVEC prevented BK/B1 induced cell growth. These results substantiate the protective and trophic role of BK on microvascular endothelium by demonstrating that Bl receptor stimulation on venular endothelium is mandatory for the angiogenic effect of BK through its coupling to NOS activation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Ahluwalia A, and Perretti M. (1999). Bl receptors as a new inflammatory target. Could this B the 1?}. TiPS20, 100–104
Bouchard J. F., Chouinard J., and Lamontagne, D. (1998) Role of kinins in endothelial protective effect of ischaemic preconditioning. Br. J. Pharmacol. 123, 413–420
Braun, C, Ade M., Unger, T, van der Woude F. J., and Rohmeiss, P. (1997) Effects of bradykinm and icatibant on renal hemodynamics in conscious spontaneously hypertensive and normotensive rats. J Cardiovasc. Pharmacol. 30, 446–454
Cambridge H., and Brain, S. D. (1995) Mechanism of bradykinin-induced plasma extravasation in the rat knee joint. Br. J Pharmacol. 115, 641–647
Cohen, M.V., and Downey, J.M. (1996). Myocardial preconditioning promises to be a novel approach to the treatment of ischemic heart disease. Annu. Rev. Med., 47, 21–29.
Granger H. J., Ziche M., Hawker, J.R. Jr, Meininger C. J., Czisny L. E., and Zawieja, D. C. (1994). Molecular and cellular basis of myocardial angiogenesis. Cell. Mol. Biol. Res, 40, 81–85.
Hall, J. M. (1992) Bradykinin receptors: pharmacological and biological roles. Pharmac Ther. 56, 131–190
Hock, F.J. Wirth K., Albus U., Linz W., Gerhards, H.J., Wiemer G., Henke, St. Breipohl G., Konig W., Knolle J., and Scholkens, B.A. (1991). Hoe 140 a new potent and long acting bradykmin-antagonist: in vitro studies. Br. J. Pharmacol., 102, 796–873.
Hu, D.-E., and Fan, T.-P. D. (1993) [Leu8]des-Arg9-bradykin?n inhibits the angiogenic effect of bradykinin and interleukin-1 in rats. Br. J. Pharmacol. 109, 14–17
Hu, D.-E., Hiley, C.R., Smither, R.L., Greesham, G.A., and T.-P.D. Fan. (1995). Methods in laboratory investigation. Colleration of 133Xe clearance, blood flow and histology in rat sponge model of angiogenesis. Lab Invest., 72, 601–610.
Morbidelli L., Parenti A., Giovannelli L., Granger H. J., Ledda F., and Ziche, M. (1998) Bl receptor involvement in the effect of bradykinin on venular endothelial cell proliferation and potentiation of FGF-2 effects. Br. J. Pharmacol. 124, 1286–1292
Noda K., Sasaguri M., Ideishi M., Ikeda M., and Arakawa, K. (1993). Role of locally formed angiotensin II and bradykinin in the reduction of myocardial infarct size in dogs. Cardiovasc Res., 27, 334–340.
Parenti A., Morbidelli L., Cui X. L., Douglas J. G., Hood J., Granger H. J., Ledda F., and Ziche, M. (1998) Nitric oxide is an upstream signal for vascular endothelial growth factorinduced extracellular signal-regulated kinases1/2 activation in postcapillary endothelium. J Biol. Chem. 273, 4220–4226
Schelhng M. E., Meininger C. J., Hawker J. R., and Granger, H. J. (1988) Venular endothelial cells from bovine heart. Am. J. Physiol. 254, H1211–H1217
Schlemper V., and Calixto, J. B. (1994) Nitric oxide pathway-mediated relaxant effect of bradykinin in the guinea-pig isolated trachea. Br. J. Pharmacol. 111, 83–88
Schriefer J. A., Broudy E. P., and Hassen, A. H. (1997). Endopeptidase inhibitors decrease myocardial ischemia/reperfusion injury in an in vivo rabbit model. J. Pharmacol. Exp. Ther., 278(3), 1034–1039.
Starkopf J., Bugge E., and Ytrehus, K. (1997). Preischemic bradykinin and ischaemic preconditioning in functional recovery of the globally ischaemic rat heart. Cardiovasc. Res, 33(l), 63–70.
Wahl, M, Whalley E. T., Unterberg A., Shilling L., Parsons A. A., Baethmann A., and Young, A. R. (1996) Vasomotor and permeability effects of bradykinin in cerebral microcirculation. Immunpharmacol. 33, 257–263
Yellon, D.M., Alkhulaifi, A.M., and Pugsley, W.B. (1993). Preconditioning the human myocardium. Lancet, 342, 276–277
Ziche M., Morbidelli L., Choudhuri R., Zhang, H.-T., Donnini S., Granger H. J., and Bicknell, R. (1997) Nitric oxide-synthase lies downstream of vascular endothelial growth factor but not basic fibroblast growth factor induced angiogenesis. J. Clin. Invest. 99, 2625–2634
Ziche M., Morbidelli L., Masini E., Amerini S., Granger H. J., Maggi C. A., Geppetti P., and Ledda L. (1994) Nitric oxide mediates angiogenesis in vivo and endothelial cell growth and migration in vitro promoted by substance P. J. Clin. Invest. 94, 2036–2044
Ziche M., Zawieja D., Hester R. K., and Granger, H. J. (1993) Calcium entry, mobilization and extrusion in postcapillary venular endothelium exposed to bradykinin. Am. J. Physiol. 34, H569–H580
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Morbidelli, L., Parenti, A., Donnini, S., Ledda, F., Granger, H.J., Ziche, M. (2000). Differential Contribution of Bradykinin Receptors in Angiogenesis. In: Maragoudakis, M.E. (eds) Angiogenesis. Advances in Experimental Medicine and Biology, vol 476. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4221-6_10
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4221-6_10
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6895-3
Online ISBN: 978-1-4615-4221-6
eBook Packages: Springer Book Archive