Summary
Cryptosporidium parvum is a well-recognized cause of diarrhea in humans and animals throughout the world, and is associated with a substantial degree of morbidity and mortality in patients with acquired immunodeficiency syndrome (AIDS). At the present time, there is no effective therapy for treating or preventing infection with C. parvum. This is primarily due to a lack of understanding of the basic cellular and molecular biology of this pathogen in terms of virulence factors, genome structure, gene expression, and regulation. Over the past few years, large-scale sequencing of randomly selected cDNAs or fragments of genomic DNA has proven to be an efficient approach for obtaining large amount of genomic information. Recently, large-scale sporozoite expressed sequence tag (EST) and genomic sequence tag (GST) projects have been initiated for C. parvum. These projects have greatly increased the number of C. parvum genes identified and demonstrate the usefulness of large-scale sequencing for expanding our understanding of C. parvum biology. Continued characterization of the C. parvum genome will increase our basic understanding of the cellular and molecular biology of C. parvum in terms of gene and genome structure, and will identify key metabolic and pathophysiologic features of the organism for future development of safe and effective strategies for prevention and treatment of disease.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Abrahamsen, M.S., Clark, T.G., Mascolo, P., Speer, C.A., and White, M.W., 1993, Developmental gene expression in Eimeria bovis, Mol. Biochem. Parasitol. 57:1–14.
Abrahamsen, M.S., Johnson, R.R., Hathaway, M., and White, M.W., 1995, Identification of Eimeria bovis merozoite cDNAs using differential mRNA display, Mol. Biochem. Parasitol. 71:183–191.
Abrahamsen, M.S., Schroeder, A.A., and Lancto, C.A., 1996, Differential mRNA display analysis of gene expression in Cryptosporidium parvum-infected HCT-8 cells, J. Euk. Microbiol. 43:80–81.
Blunt, D.S., Khramtsov, N.V., Upton, S.J., and Montelone, B.A., 1997, Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule, Clin. Diag. Lab. Immunol. 4:11–13.
Bonafonte, M.T., Priest, J.W., Garmon, D., Arrowood, M.J., and Mead, J.R., 1997, Isolation of the gene coding for elongation factor-1 alpha in Cryptosporidium parvum, Biochim. Biophys. Acta. 1351:256–260.
Campbell, P.N. and Current, W.L., 1983, Demonstration of serum antibodies to Cryptosporidium sp in normal and immunodeficient humans with confirmed infections, J. Clin. Micro. 18:165–169.
Chakrabarti, D., Reddy, G.R., Dame, J.B., Almira, E.C., Laipis, P.J., Ferl, R.J., Yang, T.P., Rowe, T.C., and Schuster, S.M., 1994, Analysis of expressed sequence tags from Plasmodium falciparum, Mol. Biochem. Parasitol. 66:97–104.
Connolly, G.M., Dryden, M.S., Shanson, D.C., and Gassard, B.G., 1988, Cryptosporidial diarrhea in Aids and its treatment, Gut 29:593–597.
Dias, R.M.D., Mangini, A.C.S., Torres, D.M.G.V., Correa, M.O.A., Lupetti, N., Correa, F.A., and Chieffe, P.P., 1988, Cryptosporidiosis among patients with acquired immunodeficiency syndrome (AIDS) in the county of Sao Paulo, Brazil, Rev. Inst. Med. Trop. Sao Paulo 30:310.
Dubey, J.P., Speer, C.A., and Fayer, R., 1990, Cryptosporidiosis of man and animals, CRC Press, Inc., Boca Raton. Fl.
El-Sayed, N.M.A., Alarcon, C.M., Beck, J.C, Sheffield, V.C., and Donelson, J.E., 1995, cDNA expressed tags of Trypanosoma brucei rhodesiense provide new insights into the biology of the parasite, Mol. Biochem. Parasitol. 73:75–90.
Fayer, R. and Ungar, B.L.P., 1986, Cryptosporidium spp. and cryptosporidiosis, Microbiol. Rev. 50:458–483.
Franco, G.R., Adams, M.D., Soares, M.B., Simpson, A.J., Venter, J.C., and Pena, S.D., 1995, Identification of new Schistosoma mansoni genes by the EST strategy using a directional cDNA library, Gene 152:141–147.
Jenkins, M.C. and C. Petersen, 1997, Molecular biology of Cryptosporidium, In: Cryptosporidium and cryptosporidiosis, Editor: Fayer, R., CRC Press, Boca Raton, Florida, p. 225–232.
Jones, D.E., Tu, T.D., Sweeney, R.W., and Clark, D.P., 1994, Isolation of Cryptosporidium and bovine cDNA clones from a Cryptosporidium-infected MDBK cell line subtraction library, J. Euk. Microbiol. 41:46–47.
Khramtsov, N.V., Oppert, B., Montelone, B.A., and Upton, S.J., 1997, Sequencing, analysis and expression in Escherichia coli of a gene encoding a 15 kDa Cryptosporidium parvum protein, Biochem. Biophys. Res. Com. 230:164–166.
Liang, P. and Pardee, A.B., 1992, Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction, Science 257:967–971.
Navin, T.R. and Harden, A.M., 1987, Cryptosporidiosis in patients with Aids, J. Infect. Dis. 155:150.
Petersen, C., 1993, Cellular biology of Cryptosporidium parvum, Parasitol. Today 9:87–91.
Schroeder, A.A., Lawrence, C.E., and Abrahamsen, M.S., 1999, Differential mRNA display cloning and characterization of a Cryptospordium parvum gene expressed during intracellular development, J. Parasitol. (in press).
Schroeder, A.A., Brown, A.M., and Abrahamsen, M.S., 1998, Identification of a developmentally-regulated Cryptosporidium parvum gene by differential mRNA display PCR, Gene 216:327–334.
Smith, P.D., Lane, H.C., Gill, V.J., Manischewitz, J.F., Quinnan, G.V., Fauci, A.S., and Masur, H., 1988, Intestinal infections in patients with the acquired immunodeficiency syndrome (AIDS): Etiology and response to therapy, Ann. Intern. Med. 108:328–333.
Spano, F., Puri, C., Ranucci, L., Putignani, L., and Crisanti, A., 1997, Cloning of the entire COWP gene of Cryptosporidium parvum and ultrastructural localization of the protein during sexual parasite development, Parasitol. 114:427–437.
Tzipori, S., 1988, Cryptosoporidiosis in perspective, Adv. Parasitol. 27:63–129.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Abrahamsen, M.S. (1999). Cryptosporidium Parvum Gene Discovery. In: Paul, P.S., Francis, D.H. (eds) Mechanisms in the Pathogenesis of Enteric Diseases 2. Advances in Experimental Medicine and Biology, vol 473. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4143-1_26
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4143-1_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6858-8
Online ISBN: 978-1-4615-4143-1
eBook Packages: Springer Book Archive