Skip to main content

Application of Near Infrared to the Analysis of Oil, Protein, Chlorophyll, and Glucosinolates in Canola/Rapeseed

  • Chapter
Canola and Rapeseed
  • 865 Accesses

Abstract

Oil, protein, chlorophyll, and glucosinolate content are quality characteristics considered, in addition to fatty acid composition, when licensing new canola/rapeseed cultivars in Canada, and as such are of primary interest to canola/rapeseed breeders. They are also the characteristics monitored throughout the canola/rapeseed transportation, processing, and marketing system. Ideally, analytical methods for these characteristics should be both reliable and economical. In addition to being accurate and precise, methods should be rapid, simple, and inexpensive since plant breeding and quality control require analysis of large numbers of samples. It is also advantageous, for plant breeding, that methods be nondestructive to allow rearing of subsequent generations. Unfortunately, all of these attributes rarely have been found in one method, and this has led to a continual evolution of analytical methodology. In recent years, near infrared (NIR) spectroscopy has been investigated as to its suitability for reliable and economical analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Appelqvist, L. A., and Johansson, S. A. 1967. Fat quality of Swedish oil crops. II. Determination of the chlorophyll content of rape, turnip rape, and white mustard seeds. J. Swed. Seed Assoc. 77: 415–431.

    Google Scholar 

  • Bengtsson, L. 1985. Improvement of rapeseed meal quality through breeding for high protein content. Dept. Crop Sci. Swed. Univ. Agric. Svalov, Sweden. 122 pp.

    Google Scholar 

  • Daun, J. K. 1976. A rapid procedure for the determination of chlorophyll in rapeseed by reflectance spectroscopy. J. Am. Oil Chem. Soc. 53: 767–770.

    Article  CAS  Google Scholar 

  • Gehrke, C. W.; Kaiser, F. E.; and Ussar, J. P. 1968. Automated method for total nitrogen, direct available P2O5 and K2O in fertilizer. Technicon Symposium. Automation in Analytical Chemistry. New York: Mediad, Inc. 1: 239–251.

    Google Scholar 

  • Heaney, R. K., and Fenwick, G. R. 1980. The analysis of glucosinolates in Brassica species using gas chromatography. Direct determination of the thiocyanate ion precursors, glucobrassicin, and neoglucobrassicin. J. Sci. Food Agric. 31: 593–599.

    Article  CAS  Google Scholar 

  • Hirschfeld, T., and Stark, E. W. 1984. Near infrared reflectance analysis of foodstuffs, in Analysis of Foods and Beverages: Modern Techniques, ed., G. Charalambous. London: Academic Press. 505–551.

    Chapter  Google Scholar 

  • Hougen, F. W.; Blank, M. J. T.; and Simpson, W. R. 1983. Sources of error in the determination of oil content of rapeseed by nuclear magnetic resonance (NMR). Proc. of 6th Int. Rapeseed Congr. Paris. May 17–19. 1,321–1,325.

    Google Scholar 

  • McGregor, D. I.; Mullin, W. J.; and Fenwick, G. R. 1983. Review of analysis of glucosinolates; analytical methodology for determining glucosinolate composition and content. J. Assoc. Off. Anal. Chem. 66: 825–849.

    CAS  Google Scholar 

  • Norris, K. H., and Hart, J. R. 1965. Direct spectrophotometric determination of moisture content in grain and seeds. Proc. of 1963 Int. Sym. on Humidity and Moisture: principles and methods of measuring moisture in liquids and solids. Humidity and Moisture. 4: 19–25.

    Google Scholar 

  • Starr, C.; Morgan, A. G.; and Smith, D. B. 1981. An evaluation of near infrared reflectance analysis in some plant breeding programs. J. Agric. Sci. Carob. 97: 107–118.

    Article  Google Scholar 

  • Starr, C.; Suttle, J.; Morgan, A. G.; and Smith, D. B. 1985. A comparison of sample preparation and calibration techniques for the estimation of nitrogen, oil, and glucosinolate content of rapeseed by near infrared spectroscopy. J. Agric. Sci. Carob. 104: 317–323.

    Article  CAS  Google Scholar 

  • Thies, W. 1974. New methods for the analysis of rapeseed constituents. Proc. of 4th Int. Rapeseed Congr. Giessen, West Germany. June 4–8. 275–282.

    Google Scholar 

  • Thies, W. 1976. Quantitative gas liquid chromatography of glucosinolates on a microliter scale. Fette Seifen Anstrichmittel. 78: 231–234.

    Article  CAS  Google Scholar 

  • Thies, W. 1977. Analysis of glucosinolates in seeds of rapeseed (Brassica napus L.): concentration of glucosinolates by ion exchange. Z. Pflanzenzuchtung 79: 331–335.

    CAS  Google Scholar 

  • Thies, W. 1978. Quantitative analysis of glucosinolates after their enzymatic desulfation on ion exchange columns. Proc. of 5th Int. Rapeseed Congr. Malmo, Sweden. June 12–16. 1: 136–139.

    Google Scholar 

  • Thies, W. 1979. Detection and utilization of a glucosinolate sulphohydrolase in the edible snail Helix pomatia. Naturwissenschaften 66: 364–365.

    Article  CAS  Google Scholar 

  • Thies, W. 1980. Analysis of glucosinolates via “on column” desulfation, in Analytical Chemistry of Rapeseed and Its Products, eds. J. K. Daun, D. I. McGregor, and E. E. McGregor. Winnipeg: Canola Council of Canada. 66–71.

    Google Scholar 

  • Thies, W., and McGregor, D. I. 1989. Analytical methods for the selection of oil content and fatty acids composition, in Oil Crops of the World, eds. G. Röbbelen, R. K. Downey, and A. Ashri. Toronto: McGraw-Hill. 132–164.

    Google Scholar 

  • Tkachuk, R. 1981. Oil and protein analysis of whole rapeseed kernels by near infrared reflectance spectroscopy. J. Am. Oil Chem. Soc. 58: 819–822.

    Article  CAS  Google Scholar 

  • Tkachuk, R.; Mellish, V. J.; Daun, J. K.; and Marci, L. J. 1988. Determination of chlorophyll in ground rapeseed using a modified near infrared reflectance spectrometer. J. Am. Oil Chem. Soc. (in press).

    Google Scholar 

  • Underhill, E. W., and Kirkland, D. F. 1971. Gas chromatography of trimethylsilyl derivatives of glucosinolates. J. Chromatogr. 57: 47–54.

    Article  CAS  Google Scholar 

  • Williams, P. C. 1975. Application of near infrared reflectance spectroscopy to analysis of cereal grains and oilseeds. Cereal Chem. 52: 561–576.

    Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer Science+Business Media New York

About this chapter

Cite this chapter

McGregor, D.I. (1990). Application of Near Infrared to the Analysis of Oil, Protein, Chlorophyll, and Glucosinolates in Canola/Rapeseed. In: Shahidi, F. (eds) Canola and Rapeseed. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3912-4_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-3912-4_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6744-4

  • Online ISBN: 978-1-4615-3912-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics