Platelet-Fibrin Interaction in the Suspension and under Flow Conditions

  • C. J. Jen
  • S. J. Hu
  • H. J. Wu
  • T. S. Lin
  • C. W. Mao
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 281)


Interactions between platelets and fibrin are important in hemostasis but often confused with platelet-fibrinogen interactions. A stirred mixture of solubilized fibrin and washed platelets at neutral pH range showed drastic reduction in turbidity and concomitant platelet adhesion onto newly formed fibrin strands. This platelet-fibrin interaction did not require platelet activation nor did it cause platelet aggregation. A device consisting of a parallel-plate flow chamber mounted on a fluorescence microscope has been constructed to allow direct visualization and recording of platelet-fibrin interaction under flow conditions. Platelets in whole blood adhered to the fibrin-coated portion but not to the uncoated portion of the flow chamber. Slow motion playback of video tapes indicated that the adhesion phenomenon was a dynamic process that involved attaching, detaching, relocation and transient contact. The fibrin coating influenced platelet adhesion both by increasing the number of cells making short-term attachments to the surface and by increasing the duration of cells attached to the surface. These observations provided basic characteristics of platelet-fibrin interaction.


Platelet Adhesion Flow Chamber Washed Platelet Platelet Deposition Adhere Platelet 
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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • C. J. Jen
    • 1
  • S. J. Hu
    • 1
  • H. J. Wu
    • 1
  • T. S. Lin
    • 2
  • C. W. Mao
    • 2
  1. 1.Department of PhysiologyNational Cheng-Kung UniversityTainanTaiwan, ROC
  2. 2.Department of Electrical EngineeringNational Cheng-Kung UniversityTainanTaiwan, ROC

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