Advertisement

Platelet-Fibrin Interaction in the Suspension and under Flow Conditions

  • C. J. Jen
  • S. J. Hu
  • H. J. Wu
  • T. S. Lin
  • C. W. Mao
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 281)

Abstract

Interactions between platelets and fibrin are important in hemostasis but often confused with platelet-fibrinogen interactions. A stirred mixture of solubilized fibrin and washed platelets at neutral pH range showed drastic reduction in turbidity and concomitant platelet adhesion onto newly formed fibrin strands. This platelet-fibrin interaction did not require platelet activation nor did it cause platelet aggregation. A device consisting of a parallel-plate flow chamber mounted on a fluorescence microscope has been constructed to allow direct visualization and recording of platelet-fibrin interaction under flow conditions. Platelets in whole blood adhered to the fibrin-coated portion but not to the uncoated portion of the flow chamber. Slow motion playback of video tapes indicated that the adhesion phenomenon was a dynamic process that involved attaching, detaching, relocation and transient contact. The fibrin coating influenced platelet adhesion both by increasing the number of cells making short-term attachments to the surface and by increasing the duration of cells attached to the surface. These observations provided basic characteristics of platelet-fibrin interaction.

Keywords

Platelet Adhesion Flow Chamber Washed Platelet Platelet Deposition Adhere Platelet 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    P. N. Walsh, Platelet-mediated coagulation protein interactions in hemostasis, Semin. Hematol.,22:178–186 (1985).PubMedGoogle Scholar
  2. 2.
    E. I. B. Peerschke, The platelet fibrinogen receptor, Semin. Hematol.,22:241–259 (1985).PubMedGoogle Scholar
  3. 3.
    G. P. Tuszynski, E. Kornecki, C. Cierniewski, L. C. Knight, A. Koshy, S. Srivastava, S. Niewiarowski, and P. N. Walsh, Association of fibrin with platelet cytoskeleton, J. Biol. Chem.,259:5247–5254 (1984).PubMedGoogle Scholar
  4. 4.
    E. J. Harfenist, M. A. Packham, and J. F. Mustard, Comparison of the interactions of fibrinogen and soluble fibrin with washed rabbit platelets stimulated with ADP, Thromb. Haemostasis, 53:183–187 (1985).Google Scholar
  5. 5.
    R. R. Hantgan, R. G. Taylor, and J. C. Lewis, Platelets interact with fibrin only after activation, Blood,65:1299–1311 (1985).PubMedGoogle Scholar
  6. 6.
    R. R. Hantgan, Fibrin protofibril and fibrinogen binding to ADP-stimulated platelets: evidence for a common mechanism, Biochim. Biophys. Acta,968:24–35 (1988).PubMedCrossRefGoogle Scholar
  7. 7.
    J. C. Lewis, C. Johnson, P. Ramsamooji, and R. R. Hantgan, Orientation and specificity of fibrin protofibril binding to ADP-stimulated platelets, Blood, 72:1992–2000 (1988).PubMedGoogle Scholar
  8. 8.
    C. J. Jen and S. J. Hu, Direct platelet-fibrin interaction that does not require platelet activation, Am. J. Physiol., 253:11745–11750 (1987).Google Scholar
  9. 9.
    Y. L. Chiu, Y. L. Chou, and C. J. Jen, Platelet deposition onto fibrin-coated surfaces under flow conditions, Blood Cells,13:437–447 (1988).PubMedGoogle Scholar
  10. 10.
    E. J. Harfenist, G. Raychaudhuri, M. A. Packham, and J. F. Mustard, An investigation into the role of coagulation factor XIII in ADP-induced aggregation and fibrinogen binding with rabbit platelets, Blood, 60:905–911 (1982).PubMedGoogle Scholar
  11. 11.
    J. A. Hubble and L. V. McIntire, Technique for visualization and analysis of mural thrombogenesis, Rev. Sci. Instrum., 57:892–897 (1986).CrossRefGoogle Scholar
  12. 12.
    I. Cohen, D. L. Burk, and J. G. White, The effect of peptides and monoclonal antibodies that bind to platelet glycoprotein IIb-IIIa complex on the development of clot tension, Blood, 73:1880–1887 (1989).PubMedGoogle Scholar
  13. 13.
    R. S. Brown, S. Niewiarowski, G. J. Stewart, and M. Millman, A double-isotope study on incorporation of platelets and red cells into fibrin, J. Lab. Clin. Med., 90:130–140 (1977).PubMedGoogle Scholar
  14. 14.
    C. J. Jen, H. J. Wu, and T. S. Lin, Direct observation of platelet adhesion onto fibrinogen-coated and fibrin-coated surfaces, Thromb. Haemostasis, 62:418 (1989).Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • C. J. Jen
    • 1
  • S. J. Hu
    • 1
  • H. J. Wu
    • 1
  • T. S. Lin
    • 2
  • C. W. Mao
    • 2
  1. 1.Department of PhysiologyNational Cheng-Kung UniversityTainanTaiwan, ROC
  2. 2.Department of Electrical EngineeringNational Cheng-Kung UniversityTainanTaiwan, ROC

Personalised recommendations