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Fibrinogen, Thrombosis, Coagulation, and Fibrinolysis pp 265–269Cite as

The Effect of Ganodermic Acid S on Human Platelets

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 281))

Summary

Incubation of gel-filtered human platelets in ganodermic acid S (lanosta-7,9(11), 24trien-3ß, 15α-diacetoxy-26-oic acid) showed that uptake of the agent by platelets was a simple diffusion process. The agent caused platelet aggregation at concentrations above 20μM. Above the threshold, the extent of cell aggregation was in a linear relationship to the agent concentration. Below the aggregation threshold, platelets showed neither the resynthesis of [32P] phosphatidylinositol 4,5-bisphosphate ([32P]PIP2) and [32P] phosphatidylinositol 4-phosphate ([32P]PIP) nor the accumulation of [32P] phosphatidic acid ([32P]PA). The results suggested that ganodermic acid S caused the activation of PIP2 hydrolysis. Scanning electron microscopy revealed that the morphology of platelets below the aggregation threshold appeared to be spiculate discoid shape. Above the threshold, the cells rounded up to spiculate irregular forms.

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Authors and Affiliations

  1. Institute of Life Science, National Tsing Hua University, Hsinchu, Taiwan, ROC

    Chuen-Neu Wang, Jia-Chyuan Chen, Ming-Shi Shiao & Cheng-Teh Wang

Authors
  1. Chuen-Neu Wang
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  2. Jia-Chyuan Chen
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  3. Ming-Shi Shiao
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  4. Cheng-Teh Wang
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Editor information

Editors and Affiliations

  1. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China

    Chung Yuan Liu

  2. University of California, San Diego, La Jolla, California, USA

    Shu Chien

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© 1990 Springer Science+Business Media New York

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Wang, CN., Chen, JC., Shiao, MS., Wang, CT. (1990). The Effect of Ganodermic Acid S on Human Platelets. In: Liu, C.Y., Chien, S. (eds) Fibrinogen, Thrombosis, Coagulation, and Fibrinolysis. Advances in Experimental Medicine and Biology, vol 281. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3806-6_27

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  • DOI: https://doi.org/10.1007/978-1-4615-3806-6_27

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6697-3

  • Online ISBN: 978-1-4615-3806-6

  • eBook Packages: Springer Book Archive

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