Skip to main content
SpringerLink
Log in
Book cover

Fibrinogen, Thrombosis, Coagulation, and Fibrinolysis pp 251–256Cite as

Studies on the Localization of Fibrinogen Binding Sites on Platelet Glycoprotein IIIa

  • Chapter

  • 205 Accesses

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 281))

Abstract

The domains of various integrins involved in the binding to adhesive proteins are poorly characterized. The approaches to localize ligand binding sites on the adhesive receptors include studies on the genetic characterization of the defective β 2 subunits in leukocyte adhesion deficiency and the defective β 1 subunit in Glanzmann thrombasthenia, studies on the chemical and photoaffinity crosslinking of the adhesive ligands to the receptors, proteolytic degradation of the integrins, attempts to recognize binding sites with monoclonal antibodies, and studies on the synthetic peptides derived from glycoprotein IIIa (GPIIIa).

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. E. I. B. Peerschke, The platelet fibrinogen receptor, Seminars in Hematology, 22:241–259 (1985).

    PubMed  CAS  Google Scholar 

  2. D. R. Phillips, I. F. Charo, L. V. Parise, and L. A. Fitzgerald, The platelet membrane glycoprotein IIb-IIIa complex, Blood, 71:831–843 (1988).

    PubMed  CAS  Google Scholar 

  3. R. O. Hynes, Integrins: A family of cell surface receptors, Cell, 48:549–554 (1987).

    Article  PubMed  CAS  Google Scholar 

  4. E. F. Plow, J. C. Loftus, E. G. Levin, D. S. Fair, D. Dixon, J. Forsyth, and M. H. Ginsberg, Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells, Proc. Nat. Acad. Sci. (USA), 83:6002–6606 (1986).

    Article  CAS  Google Scholar 

  5. E. Ruoslahti and M. D. Pierschbacher, New perspectives in cell adhesion: RGD and Integrins, Science, (Wash., DC), 238:491–497 (1987).

    Article  CAS  Google Scholar 

  6. M. E. Hemler, C. Thang, and L. Schwarz, The VLA protein family. Characterization of five distinct cell surface heterodimers each with a common 130,000 molecular weight subunit, J. Biol. Chem., 262:3300–3309 (1987).

    PubMed  CAS  Google Scholar 

  7. F. Sanchez-Madrid, J. A. Nagy, E. Robbins, P. Simm, and T. A. Springer, A human leukocyte differentiation antigen family with distinct alpha subunits and a common beta subunit: the lymphocyte-function associated antigen (LFA-1), the C3bi complement receptor (OKM1/MAC-1), and the p150, 95 molecule, J. Exp. Med.,158:1785–1803 (1983).

    Article  PubMed  CAS  Google Scholar 

  8. J. C. Giltay, and J. A. van Mourik, Structure and function of endothelial cell integrins, Haemostasis, 18:376–389 (1988).

    PubMed  CAS  Google Scholar 

  9. T. K. Kishimoto, K. O’Connor, and T. A. Springer, Leukocyte adhesion deficiency. Aberrant splicing of a conserved integrin sequence causes a moderate deficiency phenotype, J. Biol. Chem., 264:3588–3599 (1989).

    PubMed  CAS  Google Scholar 

  10. J. A. Melero and J. Gonzalez-Rodriguez, Preparation of monoclonal antibodies against glycoprotein IIIa of human platelets: Their effect on platelet aggregation, Eur. J. Biochem., 141:421–427 (1984).

    Article  PubMed  CAS  Google Scholar 

  11. J. J. Calvete, G. Rivas, M. Maurui, M. V. Alvarez, J. L. McGregor, C. L. Hew, and J. Gonzalez-Rodriguez, Tryptic digestion of human GPIIIa. Isolation and biochemical characterization of the 23 kDa N-terminal glycopeptide carrying the antigenic determinant for a monoclonal antibody (P37) which inhibits platelet aggregation, Biochem. J.,250:697–704 (1988).

    PubMed  CAS  Google Scholar 

  12. S. E. D’Souza, M. H. Ginsberg, T. A. Burke, S. C. T. Lam, and E. F. Plow, Localization of an Arg-Gly-Asp recognition site within an integrin adhesion receptor, Science,242:91–93 (1988).

    Article  PubMed  Google Scholar 

  13. J. W. Smith and D. A. Cheresh, The Arg-Gly-Asp binding domain of the vitronectin receptor. Photoaffinity crosslinking implicates amino acid residues 61–203 of the beta subunit, J. Biol. Chem., 263:18726–18731 (1988).

    PubMed  CAS  Google Scholar 

  14. J. C. Loftus, T. E. O’Toole, E. F. Plow, and M. H. Ginsberg, Identification of a GPIIa-IIIa mutation in a glanzmanns variant associated with loss of RGD binding function, Blood, 74 (suppl 1), 58a (abstract) (1989).

    Google Scholar 

  15. S. E. D’Souza, M. H. Ginsberg, and E. F. Plow, Defining the ligand recognition site within the platelet adhesion receptor GPIIb-IIIa, Blood, 74 (suppl 1), 90a (abstract) (1989).

    Google Scholar 

  16. J. Greenberg, J. L. Or, M. A. Packham, M. A. Guccione, E. J. Harfenist, R. L. Kinlough-Rathbone, D. W. Perry, and J. F. Mustard, The effect of pretreatment of human or rabbit platelets with chymotrypsin on their responses to human fibrinogen and aggregating agents, Blood, 54:753–765 (1979).

    PubMed  CAS  Google Scholar 

  17. S. Niewiarowski, A. Z. Budzynski, T. A. Morinelli, T. M. Brudzynski, and G. J. Steward, Exposure of fibrinogen receptor on human platelets by proteolytic enzymes, J. Biol. Chem.,256:917–925 (1981).

    PubMed  CAS  Google Scholar 

  18. E. Kornecki, Y. H. Ehrlich, D. D. D. D. Demars, and R. H. Lenox, Exposure of fibrinogen receptors in human platelets by surface proteolysis with elastase, J. Clin. Invest, 77:750–756 (1986).

    Article  PubMed  CAS  Google Scholar 

  19. E. Kornecki, Y. M. Ehrlich, R. Egbring, E. Gramse, R. Seitz, A. Eckardt, H. Lukasiewicz, and S. Niewiarowski, Granulocyte-platelet interactions and platelet fibrinogen receptor exposure, Amer. J. Physiol., 225:H651–H658 (1988).

    Google Scholar 

  20. E. G. Kornecki, G. P. Tuszynski, and S. Niewiarowski, Inhibition of fibrinogen receptor-mediated platelet aggregation by heterologous anti-human platelet membrane antibody: significance of an MR = 66,000 protein derived from glycoprotein IIIa, J. Biol. Chem., 285:9349–9356 (1983).

    Google Scholar 

  21. S. Niewiarowski, K. J. Norton, A. Eckardt, H. Lukasiewicz, J. C. Holt, and E. Kornecki, Structural and functional characterization of major platelet membrane components derived by limited proteolysis of glycoprotein IIIa, Biochim. Biophys. Acta, 983:91–99 (1989).

    Article  PubMed  CAS  Google Scholar 

  22. L. A. Fitzgarld, B. Steiner, S. C. Rall, S. S. Jr., Lo, and D. R. Phillips, Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone, J. Biol. Chem., 262:3936–3939 (1987).

    Google Scholar 

  23. A. B. Zimrin, R. Eisman, G. F. Vilaire, E. Schwartz, J. S. Bennett, and M. Poncz, Structure of platelet glycoprotein IIIa. A common subunit for two different membrane receptors, J. Clin. Invest., 81:1470–1475 (1988).

    Article  PubMed  CAS  Google Scholar 

  24. J. Beer and B. S. Coller, Evidence that platelet glycoprotein IIIa has a large disulfide bonded loop that is susceptible to proteolytic cleavage, J. Biol. Chem., 64:17564–17573 (1989).

    Google Scholar 

  25. S. K. A. Law, J. Gagnon, J. E. K. Hildreth, C. E. Wells, A. C. Willis, and A. J. Wong, The primary structure of the beta subunit of the cell surface adhesion glycoproteins LFA-1, CR3 and p150, 95 and its relationship to the fibronectin receptor, EMBO Journal, 6:915–919 (1987).

    PubMed  CAS  Google Scholar 

  26. J. W. Tamkun, D. W. DeSimone, D. Fonda, R. S. Patel, C. Buck, A. F. Horwitz, and R. O. Hynes, Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin, Cell,46:271–282.

    Google Scholar 

  27. K. J. Norton, J. J. Cook, and S. Niewiarowski, Effect of GPIIIa 217–232 on fibrinogen binding to human platelets, Blood,74:(suppl 1), 90a (abstract).

    Google Scholar 

  28. J. J. Cook, K. J. Norton, and S. Niewiarowski, Immunochemical characterization of GPIIIa 108–128 and GPIIIa 217–232, Blood, 74:(suppl 1), 92a (abstract).

    Google Scholar 

  29. K. J. Norton, “Structural and functional characterization of potential fibrinogen binding sites on glycoprotein IIIa of human platelets.” Dissertation submitted to the Department of Physiology, Temple University School of Medicine in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Philadelphia, PA, pp. 139 (1989).

    Google Scholar 

  30. J. S. Bennett, J. A. Hoxie, S. F. Leitman, G. Vilaire, and D. B. Cines, Inhibition of fibrinogen binding to stimulated human platelets by a monoclonal antibody, Proc. Nat. Acad. Sci., (USA), 80:2417–2421 (1983).

    Article  CAS  Google Scholar 

  31. R. L. Nachman and L. L. K. Leung, Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen, J. Clin. Invest., 69:263–269 (1982).

    Article  PubMed  CAS  Google Scholar 

  32. I. F. Charo, L. Nanninzi, D. R. Phillips, M. A. Hsu, and R. M. Scarborough, Inhibition of fibrinogen binding to GPIIb/IIIa by a GPIIIa peptide, Blood, 74:(suppl 1), 135a (abstract) (1989).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology and Thrombosis Research Center, Temple University School of Medicine, Philadelphia, PA, 19140, USA

    Stefan Niewiarowski, Karin J. Norton, Jacquelynn J. Cook & Annette Eckardt

Authors
  1. Stefan Niewiarowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin J. Norton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jacquelynn J. Cook
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Annette Eckardt
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China

    Chung Yuan Liu

  2. University of California, San Diego, La Jolla, California, USA

    Shu Chien

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer Science+Business Media New York

About this chapter

Cite this chapter

Niewiarowski, S., Norton, K.J., Cook, J.J., Eckardt, A. (1990). Studies on the Localization of Fibrinogen Binding Sites on Platelet Glycoprotein IIIa. In: Liu, C.Y., Chien, S. (eds) Fibrinogen, Thrombosis, Coagulation, and Fibrinolysis. Advances in Experimental Medicine and Biology, vol 281. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3806-6_25

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-3806-6_25

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6697-3

  • Online ISBN: 978-1-4615-3806-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation