Transformation of Prostacyclin (PGI2) to a Biologically Active Metabolite: 5(6)-Oxido-PGI1 by Cytochrome P450-Dependent Epdxygenase
The renal epoxygenase has been demonstrated to be an active pathway for the conversion of PGI2 to a new, previously unreported, metabolite. This metabolite was isolated and identified by radiogas-chromatography-mass spectrometry as 5-hydroxy-6-keto PGF1α. Its structure was further confirmed by comparison of the mass-spectra to that of the synthetic standard. The formation of 5-hydroxy-6-keto PGF1α in the kidney suggested epoxidation of prostacyclin via the renal epoxygenase as an alternative pathway of PGI2 metabolism.
KeywordsArachidonic Acid Metabolism Trimethylsilyl Ether Rabbit Kidney Microsomal Cytochrome Epoxyeicosatrienoic Acid
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