Abstract
N-Acetoxy-N-2-acetylaminofluorene, a model for the potent rat liver carcinogen N-2-acetylaminofluorene, covalently binds to the C(8) position of guanine residues. It has been shown to mainly induce frameshift mutations in the bacteria E.Coli. These mutations occur within specific DNA sequences, the so-called mutation hot-spots. Among these, the NarI sequence (GGCGCC) is especially susceptible to -2 frameshift mutations (GGCGCC -->GGCC). Due to the nature of the NarI sequence, G1G2CG3CC, three different molecular events can give rise to the observed end point GGCC (i.e. deletions of G2C, CG3 and G3C). In order to compare the actual role of each of the three possible guanine-AAF adducts in the NarI site in inducing the -2 frameshift mutation event, we constructed double stranded plasmid molecules containing a single AAF bound to one of the three guanine positions. Using these plasmids, we found that only AAF bound to G3 induces a -2 frameshift mutation event in SOS induced bacteria. This result is discussed in terms of local DNA conformation.
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Koehl, P., Burnouf, D., Fuchs, R.P.P. (1990). Mutagenesis Induced by a Single Acetylaminofluorene Adduct Within the NarI Site is Position Dependent. In: Howard, P.C., Hecht, S.S., Beland, F.A. (eds) Nitroarenes. Environmental Science Research, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3800-4_9
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DOI: https://doi.org/10.1007/978-1-4615-3800-4_9
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