Skip to main content
SpringerLink
Log in

CR1—Cytoskeleton Interactions in Neutrophils

  • Chapter
Blood Cell Biochemistry Volume 3

Part of the book series: Blood Cell Biochemistry ((BLBI,volume 3))

  • 98 Accesses

Abstract

The C3b receptor (CR1) is a polymorphic membrane glycoprotein (Dykman et al., 1983a,b, 1984, 1985; Wong et al., 1983). It is found in a variety of cell types and has several important immunologic functions (see reviews in Fearon, 1985, Schreiber, 1984, and Arnaout and Colten, 1984). For example, aside from mediating attachment of phagocytic cells to opsonized targets, erythrocyte CR1 may function in systemic clearance of complement-containing immune complexes (Cornacoff et al., 1983) or in local inhibition of complement-mediated damage to autologous tissues (Iida and Nussenzweig, 1983). It has been proposed that this function inadvertently plays a roll in the pathogenesis of CR1 deficiency found in patients with systemic lupus erythematosus (Iida et al.,1982). In addition, the human neutrophil CR1 mediates endocytosis of soluble multivalent ligand or soluble C3b-bearing complexes (Fearon et al., 1981; Abrahamson and Fearon, 1983). How this is effected has prompted researchers to investigate the relationship between CR1 and the cytoskeletal apparatus. Among the tools available to accomplish this is nonionic detergent lysis (or extraction) of cells.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abrahamson, D. R., and Fearon, D. T., 1983, Endocytosis of the C3b receptor of complement within coated pits in human polymorphonuclear leukocytes and monocytes, Lab. Invest. 48:162–168

    PubMed  CAS  Google Scholar 

  • Arnaout, M. A., and Colten, H. R., 1984, Complement C3 receptors: Structure and function, Mol. Immunol. 21:1191–1199

    Article  PubMed  CAS  Google Scholar 

  • Atkinson, J. P., Michael, J. M., Chaplin, H., Jr., and Parker, C. W., 1977, Modulation of macrophage C3b receptor function by cytochalasin-sensitive structures, J. Immunol. 118:1292–1299

    PubMed  CAS  Google Scholar 

  • Ben-Ze’ev, A., Duerr, A., Solomon, F., and Penman, S., 1979, The outer boundary of the cytoskeleton: A lamina derived from plasma membrane proteins, Cell 17:859–865

    Article  PubMed  Google Scholar 

  • Berger, M., O’Shea, J., Cross, A. S., Folks, T. M., Chused, T. M., Brown, E. J., and Frank, M. M., 1984, Human neutrophils increase expression of C3bi as well as C3b receptors upon activation, J. Clin. Invest. 74:1566–1571

    Article  PubMed  CAS  Google Scholar 

  • Berger, M., Birx, D. L., Wetzler, E. M., O’Shea, J. J., Brown, E. J., and Cross, A. S., 1985, Calcium requirements for increased complement receptor expression during neutrophil activation, J. Immunol. 135:1342–1348

    PubMed  CAS  Google Scholar 

  • Boxer, L. A., Yoder, M., Bonsib, S., Schmidt, M., Ho, P., Jersild, R., and Baehner, R. L., 1979, Effects of a chemotactic factor, N-formylmethionyl peptide, on adherence, superoxide anion generation, phagocytosis, and microtubule assembly of human polymorphonuclear leukocytes. J. Lab. Clin. Med. 93:506–514

    PubMed  CAS  Google Scholar 

  • Braun, J., Hochman, P. S., and Unanue, E. R., 1982, Ligand-induced association of surface immunoglobulin with the detergent-insoluble cytoskeletal matrix of the B lymphocyte, J. Immunol. 128:1198–1203

    PubMed  CAS  Google Scholar 

  • Brown, E. J., 1989, The interaction of small oligomers of complement 3B (C3B) with phagocytes, J. Biol. Chem. 264(11):6196–6201

    PubMed  CAS  Google Scholar 

  • Changelian, P. S., and Fearon, D. T., 1986, Tissue-specific phosphorylation of complement receptors CR1 and CR2, J. Exp. Med. 163:101–115

    Article  PubMed  CAS  Google Scholar 

  • Changelian, P. S., Jack, R. M., Collins, L. A., and Fearson, D. T., 1985, PMA induces ligand-independent internalization of CR1 on human neutrophils, J. Immunol. 134:1851–1858

    PubMed  CAS  Google Scholar 

  • Comacoff, J. B., Hebert, L. A., Smead, W. L., Van Aman, M. E., Birmingham, D. J., and Waxman, F. J., 1983, Primate erythrocyte-immune complex-clearing mechanism, J. Clin. Invest. 71:236–247

    Article  Google Scholar 

  • Dykman, T. R., Cole, J. L., Iida, K., and Atkinson, J. P., 1983a, Structural heterogeneity of the C3b/C4b receptor (CRI) on human peripheral blood cells, J. Exp. Med. 157:2160–2165

    Article  CAS  Google Scholar 

  • Dykman, T. R., Cole, J. L., Iida, K., and Atkinson, J. P., 1983b, Polymorphism of human erythrocyte C3b/C4b receptor, Proc. Natl. Acad. Sci. USA 80:1698–1702

    Article  CAS  Google Scholar 

  • Dykman, T. R., Hatch, J. A., and Atkinson, J. P., 1984, Polymorphism of the human C3b/C4b receptor: Identification of a third allele and analysis of receptor phenotypes in families and patients with systemic lupus erythematosus, J. Exp. Med. 159:691–705

    Article  PubMed  CAS  Google Scholar 

  • Dykman, T. R., Hatch, J. A., Aqua M. S., and Atkinson, J. P., 1985, Polymorphism of the C3b/C4b receptor (CRI): Characterization of a fourth allele, J. Immunol. 134:1787–1789

    PubMed  CAS  Google Scholar 

  • Fearon, D. T., 1985, Human complement receptors for C3b (CR1) and C3d (CR2), J. Invest. Dermatol. 85:55s-57s

    Article  Google Scholar 

  • Fearon, D. T., and Collins, L. A., 1983, Increased expression of C3b receptors on polymorphonuclear leu-kocytes induced by chemotactic factors and by purification procedures, J. Immunol. 130:370–375

    PubMed  CAS  Google Scholar 

  • Fearon, D. T., Kaneko, I., and Thomson, G. G., 1981, Membrane distribution and adsorptive endocytosis by C3b receptors on human polymorphonuclear leukocytes, J. Exp. Med. 153:1615–1628

    Article  PubMed  CAS  Google Scholar 

  • Flanagan, J., and Koch, G. L. E., 1978, Cross-linked surface Ig attaches to actin, Nature (London) 273:278–281

    Article  CAS  Google Scholar 

  • Fyfe, A., Hohne, E. R., Zoma, A., and Whaley, K., 1987, C3b receptor (CR1) expression on the polymorphonuclear leukocytes from patients with systemic lupus erythematosus, Clin. Exp. Immunol. 67:300–308

    PubMed  CAS  Google Scholar 

  • Griffin, F. M., Jr., and Mullinax, P. J., 1981, Augmentation of macrophage complement receptor function in vitro, J. Exp. Med. 154:291–305

    Article  PubMed  CAS  Google Scholar 

  • Hafeman, D. G., Smith, L. M., Fearon, D. T., and McConnell, H. M., 1982, Lipid monolayer-coated solid surfaces do not perturb the lateral motion and distribution of C3b receptors on neutrophils, J. Cell Biol. 94:224–227

    Article  PubMed  CAS  Google Scholar 

  • Helenius, A., and Simons, K., 1975, Solubilization of membranes by detergents, Biochim. Biophys. Acta 415:29–79

    Article  PubMed  CAS  Google Scholar 

  • Howard, T. H., and Oresajo, C. 0., 1985, The kinetics of chemotactic peptide-induced change in F-actin content, F actin distribution and the shape of neutrophils, J. Cell Biol. 101:1078–1085

    Article  PubMed  CAS  Google Scholar 

  • Iida, K., and Nussenzweig, V., 1983, Functional properties of membrane-associated complement receptor CRI, J. Immunol. 130:1876–1880

    PubMed  CAS  Google Scholar 

  • Iida, K., Momaghni R., and Nussenzweig, V., 1982, Complement receptor (CR1) deficiency in erythrocytes from patients with systemic lupus erythematosis, J. Exp. Med. 155:1427–1438

    Article  PubMed  CAS  Google Scholar 

  • Jack, R. M., and Fearon, D. T., 1983, Cytoskeletal attachment of C3b receptor (C3bR) and reciprocal co-redistribution with Fc receptor (FcR) on human polymorphonuclear leukocytes (PMN), Fed. Proc. 42:12–35

    Google Scholar 

  • Jack, R. M., and Fearon, D. T., 1984, Altered surface distribution of both C3b receptors and Fc receptors on neutrophils induced by anti-C3b receptor or aggregated IgG, J. Immunol. 132:3028–3033

    PubMed  CAS  Google Scholar 

  • Jack, R. M., Ezzell, R. M., Hartwig, I., and Fearon, D. T., 1986, Differential interaction of the C3b/C4b receptor and MHC class I with the cytoskeleton of human neutrophils, J. Immunol. 137:3996–4003

    PubMed  CAS  Google Scholar 

  • Katz, J. D., Rimmemran, C. M., Berrettoni, C. M., and Hafeman, D. G., 1987, Receptors for C3b on the neutrophil surface associate with the cytoskeleton-containing cell residue subsequent to cell lysis with nonionic detergent, J. Immunol. Methods 99:83–93

    Article  PubMed  CAS  Google Scholar 

  • Kay, A. B., Glass, E. J., and Salter, D. McG., 1979, Leukoattractants enhance complement receptors on human phagocytic cells, Clin. Exp. Immunol. 38:294–299

    PubMed  CAS  Google Scholar 

  • Kordeli, E., Cartaud, J., Nghiem, H. O., and Changeux, J. P., 1987, The torpedo electrocyte: A model system for the study of receptor-cytoskeleton interactions, J. Receptor Res. 7:71–88

    CAS  Google Scholar 

  • Malbran, A., Siwik, S., Frank, M. M., and Fries, L. F., 1988, Cr1-receptor recycling in phorbol ester-activated polymorphonuclear leukocytes, Immunology 63:325–330

    PubMed  CAS  Google Scholar 

  • Okada, K., and Brown, E. J., 1988, Sodium fluoride reveals multiple pathways for regulation of surface expression of the C3b/C4b receptor (CR1) on human polymorphonuclear leukocytes, J. Immunol. 140:878–884

    PubMed  CAS  Google Scholar 

  • Osborn, M., and Weber, K., 1977, The detergent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles, Exp. Cell Res. 106:339–349

    Article  PubMed  CAS  Google Scholar 

  • O’Shea, J. J., Brown, E. J., Seligmann, B. E., Metcalf, J. A., Frank, M. M., and Gallin, J. I., 1985a, Evidence for distinct intracellular pools of receptors for C3b and C3bi in human neutrophils, J. Immunol. 134:2580–2587

    Google Scholar 

  • O’Shea, J. J., Brown, E. J., Gaither, T. A., Takahashi, T., and Frank, M. M., 1985b, Tumor-promoting phorbol esters induce rapid internalization of the C3b receptor via a cytoskeleton-dependent mechanism, J. Immunol. 135:1325–1330

    Google Scholar 

  • O’Shea, J. J., Siwik, S. A., Gaither, T. A., and Frank, M. M., 1985c, Activation of the C3b receptor: Effect of diacylglyerols and calcium mobilization, J. Immunol. 135:3381–3387

    Google Scholar 

  • Prives, J., Fulton, A. B., Penman, S., Daniels, M. P., and Christian, C. N., 1982, Interaction of the cytoskeletal framework with acetylcholine receptor on the surface of embryonic muscle cells in culture, J. Cell Biol. 92:231–236

    Article  PubMed  CAS  Google Scholar 

  • Rao, K. M. K., and Varani, J., 1982, Actin polymerization induced by chemotactic peptide and concanavalin A in rat neutrophils, J. Immunol. 129:1605–1607

    PubMed  CAS  Google Scholar 

  • Richerson, H. B., Walsh, G. M., Walport, M. J., Moqbel, R., and Kay, A. B., 1985, Enhancement of human neutrophil complement receptors: A comparison of the rosette technique with the uptake of radio-labelled anti-CR! monoclonal antibody, Clin. Exp. Immunol. 62:442–448

    PubMed  CAS  Google Scholar 

  • Schlessinger, J., Elson, E. L., Webb, W. W., Yahara, I., Rutishauser, U., and Edelman, G. M., 1977, Receptor Diffusion on cell surfaces modulated by locally bound concanavalin A, Proc. Natl. Acad. Sci. USA 74:1110–1114

    Article  PubMed  CAS  Google Scholar 

  • Schreiber, R. D., 1984, The chemistry and biology of complement receptors, Springer Semin. Immunopathol. 7:221–249

    Article  PubMed  CAS  Google Scholar 

  • Sheterline, P., and Hopkins, C. R., 1981, Transmembrane linkage between surface glycoproteins and components of the cytoplasm in neutrophil leukocytes, J. Cell Biol. 90:743–754

    Article  PubMed  CAS  Google Scholar 

  • Sklar, L. A., Omann, G. M., and Painter, R. G., 1985, Relationship of actin polymerization and depolymerization to light scattering in human neutrophils: Dependence on receptor occupancy and intracellular Ca+ + J. Cell Biol. 101:1161–1166

    Article  PubMed  CAS  Google Scholar 

  • Tank, D. W., Wu, E., and Webb, W. W., 1982, Enhanced molecular diffusibility in muscle membrane blebs: Release of lateral constraints, J. Cell Biol. 92:207–212

    Article  PubMed  CAS  Google Scholar 

  • Turner, J. R., Tartakoff, A. M., and Berger, M., 1988, Intracellular degradation of the complement C3b/C4b receptor in the absence of ligand, J. Biol. Chem. 263:4914–4920

    PubMed  CAS  Google Scholar 

  • Wong, W. W., Wilson, J. G., and Fearon, D. T., 1983, Genetic regulation of a structural polymorphism of human C3b receptor, J. Clin. Invest. 72:685–693

    Article  PubMed  CAS  Google Scholar 

  • Wright, S. D., and Meyer, B. C., 1986, Phorbol esters cause a sequential activation and deactivation of complement receptors on polymorphonuclear leukocytes, J. Immunol. 136:1759–1764

    PubMed  CAS  Google Scholar 

  • Yu, J.,Fischman, D. A., and Steck, T. L., 1973, Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents, J. Supramol. Struct. 1:233–248

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Author notes

  1. James D. Katz

    Present address: Division of Rheumatic Diseases, Department of Medicine, The University of Connecticut Health Center, Farmington, Connecticut, 06030, USA

Authors and Affiliations

  1. Department of Medicine, The Western Pennsylvania Hospital, Pittsburgh, Pennsylvania, 15224, USA

    James D. Katz

Authors
  1. James D. Katz
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. North East Thames Regional Transfusion Centre, Brentwood, Essex, England

    J. R. Harris

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Katz, J.D. (1991). CR1—Cytoskeleton Interactions in Neutrophils. In: Harris, J.R. (eds) Blood Cell Biochemistry Volume 3. Blood Cell Biochemistry, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3796-0_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-3796-0_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6692-8

  • Online ISBN: 978-1-4615-3796-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation