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Hypoxia and Endothelial Cell Function: Alterations in Barrier and Coagulant Properties

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Book cover Atherosclerosis

Part of the book series: Altschul Symposia Series ((ALSS,volume 1))

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Abstract

Endothelial cells have a central role in regulation of vascular permeability and the coagulation mechanism1,2. Rather than passively controlling these functions, there are multiple active mechanisms through which endothelial cells contribute to the maintenance of barrier function and fluidity of blood. Control of vascular homeostasis by endothelial cells occurs in response to environmental stimuli. Recent work has focussed attention on cytokines, such as tumor necrosis factor/cachectin (TNF) and Interleukin 1, mediators of the host response which alter a range of endothelial properties and allow these cells to play a central role in the inflammatory response1.

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References

  1. Gimbrone, M. 1986. Vascular Endothelium in Hemostasis and Thrombosis, Churchill and Livingstone, New York.

    Google Scholar 

  2. Simionescu, N. and Simionescu, M. 1988. Endothelial Cell Biology. Plenum Publishing Corp., New York.

    Book  Google Scholar 

  3. Kinasewitz, G., Groome, L., Marshall, R., Leslie, W. and Diana, H. 1986. Effect of hypoxia on permeability of pulmonary endothelium of canine visceral pleura. J. Appl. Physiol. 61: 554–560.

    PubMed  CAS  Google Scholar 

  4. Olesen, S-P. 1986. Rapid increase in blood-brain barrier permeability during severe hypoxia and metabolic inhibition. Brain Res. 368: 24–29.

    Article  PubMed  CAS  Google Scholar 

  5. Sevitt, S. 1967. The acutely swollen leg and deep vein thrombosis. Brit. J. Surg. 68: 166–170.

    Google Scholar 

  6. Hamer, J., Malone, P. and Silver, I. 1981. The PO2 in venous valve pockets: its possible bearing on thrombogenesis. Brit. J. Surg. 68: 166–170.

    Article  PubMed  CAS  Google Scholar 

  7. Malone, P. 1977. A hypothesis concerning the aetiology of venous thrombosis. Med. Hypotheses 5: 189–201.

    Article  Google Scholar 

  8. Schwartz S. 1978. Selection and characterization of bovine aortic endothelial cells. In Vitro 14: 966–984.

    Article  PubMed  CAS  Google Scholar 

  9. Furie, M., Cramer, E., Naprestek, B. and Silverstein, S. 1984. Cultured endothelial monolayers that restrict the transendothelial passage of macromolecules and electrical current. J. Cell Biology 65: 1033–1042.

    Article  Google Scholar 

  10. Jaffe, E., Hoyer, L. and Nachman, R. 1973. Synthesis of antihemophilic factor antigen by cultured human endothelial cells. J. Clin. Invest. 52: 2757–2765.

    Article  PubMed  CAS  Google Scholar 

  11. Esmon, C. 1987. The regulation of natural anticoagulant pathways. Science 235: 1348–1352.

    Article  PubMed  CAS  Google Scholar 

  12. Brett, J., Gerlach, H., Nawroth, P., Steinberg, S., Godman, G. and Stern, D. 1989b. Tumor necrosis factor/cachectin increases permeability of endothelial monolayers by a mechanism involving regulatory G proteins. J. Exp. Med. 169: 1977–1991.

    Article  CAS  Google Scholar 

  13. Brett, J., Stern, D., Ogawa, S., Silverstein, S. and Loike, J. 1989a. Energy metabolism in endothelial cells: central role of glycolysis in hypoxia. J. Cell Biol. 109: 1711 (abstract).

    Article  Google Scholar 

  14. Nawroth, P. and Stern, D. 1986. Modulation of endothelial cell hemostatic properties by tumor necrosis factor. J. Exp. Med. 163: 740–745.

    Article  PubMed  CAS  Google Scholar 

  15. Gerlach, H., Liebermann, H., Brett, J., Bach, R., Godman, G. and Stern, D. 1989. Growing/motile endothelium shows enhanced responsiveness to tumor necrosis factor/cachectin. J. Exp. Med. 170: 913–931.

    Article  PubMed  CAS  Google Scholar 

  16. Madri, J., Pratt, B. and Tucker, A. 1988. Phenotypic modulation of endothelial cells by transforming growth factor-B depends upon the composition and organization of the extracellular matrix. J. Cell Biol. 106: 1375–1384.

    Article  PubMed  CAS  Google Scholar 

  17. Chirgwin, J., Przbyla, R., MacDonald, R. and Rutter, W. 1979. Isolation of biologically active ribonucleic acid from sucrose enriched ribonuclease. Biochem 18: 5294–5299.

    Article  CAS  Google Scholar 

  18. Wen, D., Dittman, W., Ye, R., Deaven, L., Majerus, P. and Sadler J. 1987. Human thrombomodulin: Complete cDNA sequence and chromosome localization of the gene. Biochem. 26: 4350–4357.

    Article  CAS  Google Scholar 

  19. Schwartzbauer, J., Tamkin, J., Lemischka, I. and Hynes R. 1983. Three different fibronectin mRNAs arise by alternative splicing within the coding region. Cell 35: 421–423.

    Article  Google Scholar 

  20. Reth, M. and Alt, F. 1984. Novel immunoglobulin heavy chains are produced from DJH gene segment rearrangements in lymphoid cells. Nature 321: 418–423.

    Article  Google Scholar 

  21. Fujikawa, K., Legaz, M. and Davie, E. 1972. Bovine factor X1 and X2. Isolation and characterization. Biochemistry 11: 4882–4891.

    Article  PubMed  CAS  Google Scholar 

  22. Fujikawa, K., Thompson, A., Legaz, M., Meyer, R. and Davie, E. 1973. Isolation and characterization of bovine factor IX. Biochemistry 12: 4938–4944.

    Article  PubMed  CAS  Google Scholar 

  23. Laemmli, U. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Article  PubMed  CAS  Google Scholar 

  24. Henderson, L. and Konigsberg, W. 1979. A micromethod for complete removal of dodecyl sulfate from proteins by ion-pair extraction. Anal. Biochem. 93: 153–157.

    CAS  Google Scholar 

  25. Lee, S-L. and Fanburg, B. 1987. Glycolytic activity and enhancement of serotonin uptake by endothelial cells exposed to hypoxia/anoxia. Circ. Res. 60: 653–688.

    Article  PubMed  CAS  Google Scholar 

  26. Ogawa, S., Gerlach, H., Esposito C., Macaulay AP., Brett, J. and Stern, D. 1989. Hypoxia modulates the barrier and coagulant properties of cultured bovine endothelium: Increased monolayer permeability and induction of procoagulant properties. J. Clin. Invest. 85: 1090–1098.

    Article  Google Scholar 

  27. Cummiskey, J., Simon, L., Theodore, J., Ryan, U. and Robin, E. 1981. Bioenergetic alterations in cultivated pulmonary artery and aortic endothelial cells exposed to normoxia and hypoxia. Exp. Lung Res. 2: 155–163.

    Article  PubMed  CAS  Google Scholar 

  28. Albelda, S., Sampson, P., Haselton, F., McNiff, J., Mueller, S., Williams, S., Fishman, A. and Levine, E. 1988. Permeability characteristics of cultured endothelial cell monolayers. J. April. Physiol. 64: 308–319.

    CAS  Google Scholar 

  29. Del Vecchio, P., Siflinger-Birnboim, A., Shepard, J., Bizions, R., Cooper, J. and Malik, A. 1987. Endothelial monolayer permeability to macromolecules. Fed. Proc. 46: 2511–2516.

    PubMed  Google Scholar 

  30. Shasby, D. and Roberts, R. 1987. Transendothelial transfer of macromolecules in vivo. Fed. Proc. 46: 2506–2512.

    PubMed  CAS  Google Scholar 

  31. Bevilacqua, M., Pober, J., Majeau, G., Cotran, R. and Gimbrone, M. 1986. Recombinant TNF induces procoagulant activity endotheliumPNAS (USA). 83: 4533–4537.

    Article  CAS  Google Scholar 

  32. Stern, D., Brett, J., Harris, K. and Nawroth, P. 1986. Participation of endothelial cells in the protein C-protein S anticoagulant pathway: The synthesis and release of protein S.J. Cell Biol. 102: 1971–1978.

    Article  PubMed  CAS  Google Scholar 

  33. Clark, M., Chen, M-J., Crooke, S. and Bomalaski, J. 1988. Tumor necrosis factor (cachectin) induces phospholipase A2-activating protein in endothelial cellsBiochem. J. 250: 125–132.

    PubMed  CAS  Google Scholar 

  34. Wojta, J., Jones, R., Binder, B., and Hoover, R. 1988. Reduction in PO2 decreases the fibrinolytic potential of cultured bovine endothelial cells derived from pulmonary arteries and lung microvasculatureBlood. 71: 1703–1706.

    PubMed  CAS  Google Scholar 

  35. Falanga, A. and Gordon, S. 1985. Isolation and characterization of cancer procoagulant: A cysteine proteinase from malignant tissueBiochemistry. 24: 5558–5567.

    Article  PubMed  CAS  Google Scholar 

  36. Anderson, G., Stoler, D. and Scarcello, L. 1989. Normal fibroblasts responding to anoxia exhibit features of the malignant phenotypeJ. Biol. Chem. 264: 14885–14892.

    PubMed  CAS  Google Scholar 

  37. Sciandra, J., Subjeck, J. and Hughes, H. 1984. Induction of glucose-regulated proteins during anaerobic exposure and of heat-shock proteins after reoxygenationPNAS (USA). 81: 4843–4847.

    Article  CAS  Google Scholar 

  38. Subjeck, J. and Thung-Tai, S. 1986. Stress protein systems of mammalian cellsAm. J. Physiol. 250: C1–C17.

    PubMed  CAS  Google Scholar 

  39. Wilson, R. and Sutherland, R. 1989. Enhanced synthesis of specific proteins, RNA and DNA caused by hypoxia and reoxygenationJ. Radiation Oncology Biol. Phys. 16: 957–961.

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons of Columbia University, 630 West 168th Street, New York, NY, 10032, USA

    Satoshi Ogawa, Masayasu Matsumoto, Jerold Brett, Matthias Clauss & David M. Stern

  2. First Department of Medicine, Osaka University Medical School Hospital, Osaka, 553, Japan

    Masayasu Matsumoto

Authors
  1. Satoshi Ogawa
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  2. Masayasu Matsumoto
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  3. Jerold Brett
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  4. Matthias Clauss
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  5. David M. Stern
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Editor information

Editors and Affiliations

  1. University of Toronto, Toronto, Ontario, Canada

    Avrum I. Gotlieb  & B. Lowell Langille  & 

  2. University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Sergey Fedoroff

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© 1991 Springer Science+Business Media New York

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Ogawa, S., Matsumoto, M., Brett, J., Clauss, M., Stern, D.M. (1991). Hypoxia and Endothelial Cell Function: Alterations in Barrier and Coagulant Properties. In: Gotlieb, A.I., Langille, B.L., Fedoroff, S. (eds) Atherosclerosis. Altschul Symposia Series, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3754-0_14

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  • DOI: https://doi.org/10.1007/978-1-4615-3754-0_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6672-0

  • Online ISBN: 978-1-4615-3754-0

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