Abstract
Phytochrome is the photochromic receptor for a variety of photo-morphogenic and developmental responses of higher plants to red light (for a detailed review of the subject, see Song 1983,Song 1988; Lagarias, 1985; Quail et al., 1986; Schaefer and Briggs, 1986; Furuya, 1987). In this paper, results on the study of the role of the chromophore on the phototransformation of 124 kDa oat phytochrome obtained in our laboratory will be reviewed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Chai, Y.G., Song, P.S., Cordonnier, M.M. and Pratt, L.H. (1987) A photoreversible circular dichroism spectral change in oat phytochrome is suppressed by a monoclonal antibody that binds near its N-terminus and by chromophore modification. Biochemistry 26: 4947–4952.
Choi, J.K. (1987) Protein topography and binding properties of phytochrome. Ph. D. Dissertation, Texas Tech University, Lubbock, Texas.
Cordonnier, M.M., Greppin, H. and Pratt, L.H. (1985) Monoclonal antibodies with differing affinities to the red-absorbing and far-red absorbing forms of phytochrome. Biochemistry 24: 3246–3253.
Farrens, D., Song, P.S., Ruediger, W. and Eilfeld, P. (1989) J. Plant Physiol., 134:269–275.
Furuya, M. (Ed.) (1987) Phytochrome and Photoregulation in Plants, Academic Press, Tokyo and New York.
Grimm, R., Eckerskorn, C., Lottspeich, F., Zenger, C. and Ruediger, W. (1988) Sequence analysis of proteolytic fragments of 124-kilodalton phytochrome from etiolated Avena sativa L.: Conclusions on the conformation of the native protein. Planta 174: 396–401.
Hahn, T.R., Song, P.S., Quail, P.H. and Vierstra, R.D. (1984) Tetranitromethane oxidation of phytochrome chromophore as a function of spectral form and molecular weight. Plant Physiol. 74: 755–758.
Hahn T.R. and Song, P.S. (1981) Hydrophobic properties of phytochrome as probed by Banilinonaphthalene-l-sulfonate fluorescence. Biochemistry 20: 2602–2609.
Holdsworth, M.L. and Whitelam, G.C. (1987) A monoclonal antibody specific for the red-absorbing form of phytochrome. Planta 172: 539–547.
Kim, I.S., Bai, U. and Song, P.S. (1988) The 124-kDa oat phytochrome does not possess protein kinase activity. Photochem. Photobiol., 49:319–323.
Lagarias, J.C. (1985) Progress in the molecular analysis of phytochrome. Photochem. Photobiol. 42: 811–820.
Lagarias, J.C. and Mercurio, F.M. (1985) Structure-function studies of phytochrome. Identification of light-induced conformational changes in 124-kDa Avena phytochrome in vitro. J. Biol. Chem. 260: 2415–2423.
Lagarias, J.C., Wong, Y.S., Berkelman, T.R., Kidd, D.G. and McMichael, Jr. R.W. (1987) Structure-function studies on Avena phytochrome. In: Phytochrome and Photoregulation in Plants (M. Furuya, Ed) Academic Press, Tokyo and New York, pp. 51–61.
Lisansky, S.G. and Galston, A.W. (1974) Phytochrome stability in vitro. 1. Effect of metal ions. Plant Physiol. 53: 352–359.
Quail, P.H., Colbert, J.T., Peters, N.K., Christensen, A.H., Sharrock, R.A. and Lissemore, J.L. (1986) Phytochrome and the regulation of the expression of its genes. Phil. Trans. R. Soc. London,B314: 469–480.
Ruediger, W., Thuemmler, F., Cmiel, E. and Schneider, S. (1983) Chromophore structure of the physiologically active form (Pfr) of phytochrome. Proc. Natl. Acad. Sci. USA, 80: 6244–6248.
Schaefer, E. and Briggs, W.R. (1986) Photomorphogenesis from signal perception to gene expression. Photobiochem. Photobiophys. 12: 305–320.
Song, P.S. and Sommer, D. (1990) The chromophore topography and scondary structure of 124-kDA Avena Phytochrome probed by Zn2+-induced chromophore modification. Biochemistry,29:1934–1948.
Song, P.S. (1983) Protozoan and related photoreceptors: molecular aspects. Annu. Rev. Biophys. Bioengin. 12: 35–68.
Song, P.S. (1988) The molecular topography of phytochrome: Chromophore and apoprotein. J. Photochem. Photobiol. Pt B, 2:43–57.
Song, P.S. and Yamazaki, I. (1987) Structure-function relationship of the phytochrome chromophore. In: Phytochrome and Photoregulation in Plants (M. Furuya, Ed), Tokyo and New York, pp. 139–156.
Thuemmler, F., Eilfeld, P., Ruediger, W., Moon, D.K. and Song, P.S. (1985) On the chemical reactivity of phytochrome chromophore in the Pr and Pfr form. Z. Naturforsch. 40 c: 215–218.
Vierstra, R.D. and Quail, P.H. (1983) Purification and initial characterization of 124 kilodalton phytochrome from Avena. Biochemistry, 22: 2498–2505.
Wong, Y.S., Cheng, H.C., Walsch, D.A. and Lagarias, J.C. (1986) Phosphorylation of Avena phytochrome in vitro as a probe of light-induced conformation changes. J. Biol. Chem. 261: 12089–12097.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Song, PS., Bai, U., Kim, IS., Whitelam, G.C., Markwell, J.P. (1991). The Chromophore and its Role in the Phototransformation of Phytochrome: Conformational Changes Probed with bis-ANS, Monoclonal Antibodies and Phosphorylation. In: Riklis, E. (eds) Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3732-8_43
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3732-8_43
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6661-4
Online ISBN: 978-1-4615-3732-8
eBook Packages: Springer Book Archive