Abstract
A variety of dicarboxylic acids (Breatnach, 1984;Hsu, 1986;Passy, 1984;Picardo, 1983;Robbins, 1985;Schachtshabel et al., 1986), such as azelaic acid and sebacic acid have been shown to be cytotoxic for melanoma cells with varying degrees of effectiveness. The mechanism of action of these drugs remains to be elucidated, but evidence from electron microscopic studies points to the mitochondria as a primary target of dicarboxylic acids. The microspectrofluorometric approach (Hirschberg, 1978;Kohen et al., 1986) which we have developed opens the possibility to investigate the intracellular actions of azelaic acids and other such compounds in situ at the level of organelles and metabolic compartments. An illustrative use of this method has already been made in connection with another dermatological affection: a microspectrofluorometric study was carried out recently on the effect of anthralin (Kohen et al., 1986), an antipsoriatic drug, on cellular structures and metabolism.
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References
Breatnach, A.S., 1984 Brit. J. Dermatol. 111: 115–120.
Conover, T.E. and Siebert, G., 1965 Biochim. biophys. Acta 99: 1–12.
Georgiev, G.P., 1967 Enzyme Cytology edited by D.B. Roodyn (London and New York: Academic Press), pp. 27–102.
Goldstein, S. and Korczack, L.B., 1981 J. Cell Biol. 91: 392–398.
Hirschberg, J.G., Wouters, A.W., Kohen, E., Thorell, B., Eisenberg, B., Salmon, J.M. and Ploem, J.S., 1978 Multichannel Image Detectors edited by Y. Talmi (Washington, D.C.: American Chemical Society Symposium), pp. 263–289.
Hsu, F., 1986 Brit J. Dermatol. 114: 17–26.
Kohen, E., Hirschberg, J.G., Fried, M., Kohen, C., and Prince, 1988 Chemical Carcinogens edited by P. Politzer and F.J. Martin Jr. (Amsterdam: Elsevier), pp. 345–361.
Kohen, E., Hirschberg, J.G., Fried, M., Kohen, C., Santus, R., Reyftmann, J.P., Morliere, P., Schachtschabel, D.O., Mangel, W.F., Shapiro B.L., and Prince, J., 1987 Microbeam Analysis edited by Roy H. Geiss (San Francisco: San Francisco Press), pp. 231–240.
Kohen, E., Kohen, C., Hirschberg, J.G. and Schachtschabel, D.O. 1989 Cell Structure and Function by Microspectrofluorometry edited by E. Kohen, J.G. Hirschberg and J.S. Ploem (New York Academic Press), pp. 199–228.
Kohen, E., Kohen, C., Hirschberg, J.G., Wouters, A.W., Bartick, P.R., Thorell, B., Bereiter-Hahn, J., Meda, P., Rabinovitch, A., Mintz, D. and Ploem, J.S., 1982 Techniques in Cellular Physiology. Part I, edited by P.F. Baker (Amsterdam: Elsevier Biomedical), pp. P103/1–28.
Kohen, E., Kohen, C. and Jenkins, W., 1966 Exptl Cell Res. 44: 175–194.
Kohen, E., Kohen, C., Morliere, P., Santus, R., Reyftmann, J.P., Dubertret, L., Hirschberg, J.G. and Coulomb, B., 1986b Cell Biochemistry and Function 4: 157–168.
Kohen, E., Prince, J., Kohen, C., Hirschberg, J.G., and Fried, M., (1988). Time-Resolved Laser Spectroscopy in Biochemistry SPIE Vol. 909 (Bellingham, WA: The Society of Photo-Optical Instrumentation Engineering), pp. 231–240.
Kohen, E., Welch, G.R., Kohen, C., Hirschberg, J.G. and Bereiter-Hahn, J. (1986b). The Organization of Cell Metabolism edited by J.S. Clegg and G.R. Welch (New York: Plenum), pp. 251–275.
Meiskens, F., personal communication.
Passy, B.S., 1984 Biochem. Pharmacol. 33: 103–108.
Picardo, M., 1983 J. Investig. Dermatol. 80: 350.
Robbins, E.J., 1985 J. Investig. Dermotol. 80: 216–222.
Schachtshabel, D.O., Thome, D., Salzer, V., 1986 J. Invest. Dermatol. 87: 423 Abstract
Shapiro, B.L., 1979 Nature 278: 276–277.
Shapiro, B.L., Lam, L.F. and Feigal, R.J., 1982 Amer. J. Human Genet. 34: 846–852.
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Kohen, E., Kohen, C., Schachtschabel, D.O., Hirschberg, J.G., Shapiro, B.L., McHeileh, A. (1991). Microspectrofluorometry of Human Melanoma Cells and Fibroblasts Treated with Azelaic Acid. In: Riklis, E. (eds) Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3732-8_34
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DOI: https://doi.org/10.1007/978-1-4615-3732-8_34
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