Abstract
There has been considerable recent interest in the use of in vitro techniques for studying the neoplastic transformation of mammalian cells. These are techniques whereby cells of normal growth characteristics and morphology may be converted to those with characteristics of tumor cells including the ability to form invasive cancers in syngeneic hosts. Most quantitative studies of transformation have been carried out with established mouse embryo-derived cell lines, notably the C3H10T1/2 and BALB/3T3 cell systems. Although these cell lines are already immortal and aneuploid, they can be transformed to fully malignant, tumorigenic cells in vitro by exposure to various physical and chemical carcinogens.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Cadet, J., Shaw, A., Voituriez, L. and Kan, L.-S. (1989) Comparative effects of ultraviolet and ionizing radiation on nucleic acids (This Symposium).
Chan, G. and Little, J.B. (1976) Induction of oncogenic transformation in vitro by ultraviolet light. Nature 264: 442–444.
Chan, G. L. and Little, J.B. (1978) Induction of ouabain resistant mutations in C3H 10T’ cells by ultraviolet light. Proc. Natl. Acad. Sci. U.S.A. 75: 3363–3366.
Chan, G. L. and Little, J.B. (1982a) Modulation of survival and transformation during plateau-phase holding of UV-irradiated mouse cells. Mutation Res. 104: 183–186.
Chan, G. L. and Little, J.B. (1982b) Dissociated occurrence of single-gene mutation and oncogenic transformation in C3H 10T’ cells exposed to ultraviolet light and caffeine. J. Cell Physiol. 111: 309–314.
Evans, H.H. (1989) Mutagenic effecgts of ultraviolet and ionizing radiation. (This Symposium).
Fernandez, A., Mondal, S. and Heidelberger, C. (1980) Probabilistic view of the transformation of cultured C3H/10T 1/2 cells by 3-methylcholanthrene. Proc. Natl. Acad. Sci. USA 77, 7272–7276.
Fornace, A. J., Jr., Nagasawa, H. and Little, J.B. (1980) Relationship of DNA repair to chromosome aberrations, sister chromatid exchanges and survival during liquid holding recovery in X-irradiated mammalian cells. Mutat. Res. 70: 323–336.
Grisham, J.W., Smith, G.J., Lee, L.W., Bentley, K.S. and Fatteh, M.V. (1988) Spontaneous formation of foci of morphologically transformed cells in populations of C3H 10T 1/2 (clone 8) cells. Cancer Res. 48, 5969–5976.
Kennedy, A. R., Mondal, S., Heidelberger, C. and Little, J.B. (1978) Enhancement of X-ray transformation by 12–0-tetradecanoyl-phorbol-13-acetate in a cloned line of C3H mouse embryo cells. Cancer Res. 38: 439–443.
Kennedy, A. R., Murphy, G. and Little, J.B. (1980a) The effect of time and duration of exposure to 12–0-tetradecanoyl-phorbol-13-acetate (TPA) on X-ray transformation of C3H 10T’ cells. Cancer Res. 40: 1915–1920.
Kennedy, A. R., Fox, M., Murphy, G. and Little, J.B. (1980b) Relationship between X-ray exposure and malignant transformation in C3H 10T’ cells. Proc. Natl. Acad. Sci. U.S.A. 77: 7262–7266.
Kennedy, A. R. and Little, J.B. (1980) Investigation of the mechanism for enhancement of radiation transformation in vitro by 12–0-tetradecanoylphorbol-13-acetate. Carcinogenesis 1: 1039–1047.
Kennedy, A. R., Cairns, J. and Little, J.B. (1984) Timing of the steps in transformation of C3H 10T’ cells by X-irradiation. Nature 307: 85–86.
Leenhouts, H.P. and Chadwick, K.H. (1989) A molecular model for the cytotoxic action of UV and ionizing radiation (This Symposium).
LeMotte, P. K., Adelstein, S.J. and Little, J.B. (1982) Malignant transformation induced by incorporated radionuclides in BALB/3T3 mouse embryo fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 79: 7763–7767.
Liber, H. L., Leong, P.H., Terry, V.H. and Little, J.B. (1986) X-rays mutate human lymphoblast cells at genetic loci that should respond only to point mutagens. Mutat. Res. 163: 91–97.
Little, J.B. and Krolewski, B. Radiation transformation and oncogene activation. In: Primary Changes and Control Factors in Carcinogenesis, T. Friedberg and F. Oesch, eds., Deutscher Fachschriften-Verlag, Wiesbaden, Germany, 1986, pp.107–112.
Little, J.B., Yandell, D.W. and Liber, H.L. (1987) Molecular analysis of mutations at the tk and hgprt loci in human cells. Banbury Report 28, Mammalian Cell Mutagenesis, Cold Spring Harbor Laboratory, pp. 235–236.
Mondal, S. and Heidelberger, C. (1976) Transformationof C3H/10T 1/2 C18 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260, 710–711.
Nagasawa, H., Fornace, A.J. Jr., Ritter, M.A. and Little, J.B. (1982) Relationship of enhanced survival during confluent holding recovery in ultraviolet-irradiated human and mouse cells to chromosome aberrations, sister chromatid exchanges, and DNA repair. Radiat. Res. 92: 483–496.
Reznikoff, C.A., Bertram, J.S., Brankow, D.W. and Heidelberger, C. (1973) Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33, 3239–3249.
Stomato, T., Weinstein, R., Peters, B., Hu, J., Doherty, B. and Giaccia, A. Delayed mutation in Chinese hamster cells. Somatic Cell Molec. Gen. 13, 57–66.
Terzaghi, M. and Little, J.B. (1975) Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548–549.
Terzaghi, M. and Little, J.B. (1976) X-radiation induced transformation in a C3H mouse embryo-derived cell line. Cancer Res. 36: 1367–1374.
Yandell, D. W., Dryja, T.P. and Little, J.B. (1986) Somatic mutations at a heterozygous autosomal locus in human cells occur more frequently by allele loss than by intragenic structural alterations. Somatic Cell and Molecular Genetics 12: 2 55–263.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Little, J.B. (1991). Malignant Transformation by Ultraviolet and Ionizing Radiations. In: Riklis, E. (eds) Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3732-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3732-8_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6661-4
Online ISBN: 978-1-4615-3732-8
eBook Packages: Springer Book Archive