Abstract
Several authentic bile acids and related compounds were tested for their ability to lyse the cells of a halophilic archaeobacterium Halobacterium cutirubrum. They showed different lytic activities depending on the structure (number and configuration of OH group). For example, chenodeoxycholic acid caused complete lysis of the cells in a few minutes, while ursodeoxycholic acid showed very little activity. Bile acids which showed no lytic activity did not cause any decrease of viable cell numbers of H. cutirubrum by incubation for 10 minutes.
A proteolytic enzyme F-II of an unidentified halophilic archaeobacterium 172 P1 caused lysis of all tested strains of halobacteria at a concentration of 1-1.5 mg/ml in the presence of 31% NaC1, though at varying degree from strain to strain. Subtilisin Carlsberg also showed slight lytic activity on H. cutirubrum under similar conditions. Another protease preparation of Haloferax mediterranei caused spheroplast formation of some strains of halobacteria, while some were not affected.
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© 1991 Springer Science+Business Media New York
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Kamekura, M., Seno, Y. (1991). Lysis of Halobacteria with Bile Acids and Proteolytic Enzymes of Halophilic Archaeobacteria. In: Rodriguez-Valera, F. (eds) General and Applied Aspects of Halophilic Microorganisms. NATO ASI Series, vol 201. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3730-4_43
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DOI: https://doi.org/10.1007/978-1-4615-3730-4_43
Publisher Name: Springer, Boston, MA
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