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The Expression of Therapeutic Proteins in Transgenic Animals

  • Chapter
Recombinant Technology in Hemostasis and Thrombosis

Abstract

Human plasma has traditionally been a source of therapeutic proteins. Hemostatic proteins sucu as factors VIII, IX, and X, as well as antithrombin III, albumin, immunoglobulins, fibrinogen and protein C are routinely isolated from human plasma. Improved protein isolation and viral inactivation techniques have led to the production of purer and safer plasma derivatives, although in some cases in quantities insufficient to meet patient needs.

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References

  1. Anson DS, Austen DEG, Brownlee GG: Expression of active human clotting factor IX from recombinant DNA clones in mammalian cells. Nature 315: 683, 1985

    Article  PubMed  CAS  Google Scholar 

  2. Kaufman RJ, Wasley LC, Furie BC, Furie B, Shoemaker CB: Expression, purification and characterization of recombinant gamma-carboxylated Factor IX synthesis in Chinese hamster ovary cells. J Biol Chem 261: 9622, 1986

    PubMed  CAS  Google Scholar 

  3. Kaufman RJ, Wasley LC, Dorner AJ: Synthesis, processing, and secretion of recombinant human factor VIII expressed in mammalian cells. J Biol Chem 263: 6352, 1988

    PubMed  CAS  Google Scholar 

  4. Sarver N, Ricca GA, Link J, Nathan MH, Newman J, Drohan WN: Stable expression of recombinant Factor VIII molecules using a bovine papillomavirus vector. DNA 6: 553, 1987

    Article  PubMed  CAS  Google Scholar 

  5. Clark AJ, Simons P, Wilmut I, Lathe R: Pharmaceuticals from transgenic livestock. Tibtech 5: 20, 1987

    Article  CAS  Google Scholar 

  6. Jaenish R: Transgenic animals. Science 240: 1468, 1988

    Article  Google Scholar 

  7. Hammer RE, Pursel VG, Rexroad CF, Wall RJ, Bolt DJ, Palmiter RD, Brinster RL: Genetic engineering of mammalian embryos. J Anim Sci 63: 269, 1986

    PubMed  CAS  Google Scholar 

  8. Gordon JW, Ruddle FH: Integration and stable germ line transmission of genes injected into mouse pronuclei. Science 214: 1244, 1981

    Article  PubMed  CAS  Google Scholar 

  9. Brinster RL, Chen HY, Trumbauer ME, Yagle MK, Palmiter RD: Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs. Proc Natl Acad Sci 82: 4438, 1985

    Article  PubMed  CAS  Google Scholar 

  10. Lee K-F, Atiee SH, Rosen JM: Differential regulation of rat beta-casein chloramphenicol acetyltransferase fusion gene expression in transgenic mice. Mol Cell Biol 9: 560, 1989

    PubMed  CAS  Google Scholar 

  11. Behringer RR, Hammer RE, Brinster, RL, Palmiter RD, Townes TM: Two 3′sequences direct adult erythroid-specific expression of human beta-globin genes in transgenic mice. Proc Natl Acad Sci USA 84: 7056, 1987

    Article  PubMed  CAS  Google Scholar 

  12. Brinster RL, Allen JM, Behringer, RR, Gelinas RE, Palmiter RD: Introns increse transcriptional efficiency in transgenic mice. Proc Natl Acad Sci USA 85: 836, 1988

    Article  PubMed  CAS  Google Scholar 

  13. Buchman AR, Berg P: Comparison of Intron-Dependent and Intron-Independent Gene Expression. Mol Cell Biol 8: 4395, 1988

    PubMed  CAS  Google Scholar 

  14. Carlberg K, Ryden TA, Beemon K: Localization and footprinting of an enhancer within the Avian Sarcoma virus gag gene. J. Virol 62: 1617, 1988

    PubMed  CAS  Google Scholar 

  15. van Assendelft GB, Hanscombe O, Grosveld F, Greaves DR: The beta-globin dominant control region activates homologous and heterologous promoters in a tissue-specific manner. Cell 56: 969, 1989

    Article  Google Scholar 

  16. Behringer RR, Ryan TM, Reilly MP, Asakura T, Palmiter RD, Brinster RL, Townes TM: Synthesis of functional human hemoglobin in transgenic mice. Science 245: 971, 1989

    Article  PubMed  CAS  Google Scholar 

  17. Lang G, Wotton D, Owen MJ, Sewell WA, Brown MH, Mason DY, Crumpton MJ, Kioussis D: The structure of the human CD2 gene and its expression in transgenic mice. EMBO J 7: 1675, 1988

    PubMed  CAS  Google Scholar 

  18. Pittius CW, Hennighausen L, Lee E, Westphal H, Nicois E, Vitale J, Gordon K: A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. Proc Natl Acad Sci 85: 5874, 1989

    Article  Google Scholar 

  19. Pinkert CA, Ornitz DM, Brinster RL, Palmiter RD: An albumin enhancer located 10 kb upstream functions along with its promoter to direct efficient, liver-specific expression in transgenic mice. Genes Devel 1: 268, 1987

    Article  PubMed  CAS  Google Scholar 

  20. Grosveld F, van Assendelft GB, Greaves DR, Kollias G: Position-independent, high-level expression of the human beta-globin gene in transgenic mice. Cell 51: 975, 1987

    Article  PubMed  CAS  Google Scholar 

  21. Lee K-F, DeMayo, FJ, Atiee SH, Rosen JM: Tissue-specific expression of the rat beta-casein gene in transgenic mice. Nuc Ac Res 16: 1027, 1988

    Article  CAS  Google Scholar 

  22. Bayna EM, Rosen JM: Tissue specific, high level expression of rat whey acidic protein gene in transgenic mice. Nuc Ac Res 18: 2977, 1990

    Article  CAS  Google Scholar 

  23. Tomasetto C, Wolf C, Rio MC, Mehtali M, LeMeur M, Gerhinger P, Chambon P, Lathe R: Breast cancer protein PS2 synthesis in mammary gland of transgenic mice and secretion into milk. Mol Endocrinol 3: 1579, 1989

    Article  PubMed  CAS  Google Scholar 

  24. Yu SH, Deen KC, Lee E, Hennighausen L, Sweet RW, Rosenberg M, Westphal, H: Functional human CD4 protein produced in milk of transgenic mice. Mol Biol Med 6: 255, 1989

    PubMed  CAS  Google Scholar 

  25. Simons JP, McClenaghan M, Clark AJ: Alteration of the quality of milk by expression of sheep beta-lactoglobulin in transgenic mice. Nature 328: 530, 1987

    Article  PubMed  CAS  Google Scholar 

  26. Archibald AL, McClenaghan M, Homsey V, Simons JP, Clark AJ: High level expression of biologically active human alpha-1-antitrypsin in the milk of transgenic mice. Proc Natl Acad Sci USA 87: 5178, 1990

    Article  PubMed  CAS  Google Scholar 

  27. Clark AJ, Bessos H, Bishop JO, Brown P, Harris S, Lathe R, McClenaghan M, Prowse C, Simons JP, Whitelaw CBA, and Wilmut I: Expression of human anti-hemophilic factor IX in the milk of transgenic sheep. Biotechnology 7, 1989

    Google Scholar 

  28. Hall L, Emery DC, Davies MS, Parker D, Craig RK: Organization and sequence of the human alpha-lactalbumin gene. Biochem J 242: 735, 1987

    PubMed  CAS  Google Scholar 

  29. Lubon H, Hennighausen L: Conserved region of the rat alpha-lactalbumin promoter is a target for protein binding in vitro. Biochem J 256: 391, 1988

    PubMed  CAS  Google Scholar 

  30. Piletz JE, Heinleu M, Ganshow RE: Biochemical characterization of a novel whey protein from murine milk. J Biol Chem 256: 11509, 1981

    PubMed  CAS  Google Scholar 

  31. Hennighausen LG Sippel AE: Comparitive sequence analysis of the mRNAs coding for mouse and rat whey proteins. Nuc Ac Res 10: 3733, 1982

    Article  CAS  Google Scholar 

  32. Campbell SM, Rosen JM, Hennighausen LG, Strech-Jurk U, Sippel AE: Comparison of the whey acidic protein genes of the rat and the mouse. Nuc Ac Res 12: 8685, 1984

    Article  CAS  Google Scholar 

  33. Hobbs AA, Richards DA, Kessler DJ Rosen JM: Complex hormonal regulation of rat casein gene expression. J Biol Chem 257: 3598, 1982

    PubMed  CAS  Google Scholar 

  34. Richards DA, Rodgers JR, Supowit SC, Rosen JM: Construction and preliminary characterization of the rat casein and lactalbumin cDNA clones. J Biol Chem 256: 526, 1981

    PubMed  CAS  Google Scholar 

  35. Andres A-C, Schoenenberger C-A, Groner B, Hennighausen L, LeMeur M, Gerlinger P: Ha-ras oncogene expression by a milk protein gene promoter: tissue specificity, hormonal regulation and tumor induction in transgenic mice. Proc Natl Acad Sci USA 84: 1299, 1987

    Article  PubMed  CAS  Google Scholar 

  36. Schoenenberger C-A, Andres A-C, Groner B, van der Valk M, LeMeur M, Gerlinger P: Targeted c-myc expression in mammary glands of transgenic mice induces mammary tumors with constitutive milk protein gene transcription. EMBO J 7: 169, 1988

    PubMed  CAS  Google Scholar 

  37. Pittius CW, Sankaran L, Topper YL, Hennighausen L: Comparison of the regulation of the whey acidic protein gene with that of a hybrid gene containing the whey acidic gene promoter in transgenic mice. Mol Endocrinol 2: 1027, 1988b

    Article  PubMed  CAS  Google Scholar 

  38. Gordon K, Lee E, Vitale JA, Smith AE, Westphal H, Hennighausen L: Production of human tissue plasminogen activator in transgenic mouse milk. Biotech 5: 1183, 1987

    Article  CAS  Google Scholar 

  39. Craig RK, Perera PAJ, Mellor A, Smith A: Initiation and processing in vitro of the primary translational product of guinea-pig caseins. Biochem J 184: 261, 1979

    PubMed  CAS  Google Scholar 

  40. Church RB: Can. Pacific Biotech Symp Agric and Forest Bull 8: 22–23, 1986

    Google Scholar 

  41. Simons JP, Land RB: Transgenic Livestock. J Reprod Fert Suppl 34: 237, 1987

    CAS  Google Scholar 

  42. Church RB: Embryo manipulation and gene transfer in domestic animals. Tibtech 5: 13–19, 1987

    Article  Google Scholar 

  43. Hammer RE, Pursel VG, Rexroad Jr CE, Wall RJ, Bolt DJ, Ebert KM, Palmiter RD, Brinster RL: Production of transgenic rabbits, sheep and pigs by microinjection. Nature 315: 680, 1985

    Article  PubMed  CAS  Google Scholar 

  44. Wong NP, ed.: Fundamentals of Dairy Chemistry, 3rd Edition. New York, NY, Van Nostrand Reinhold Co., 1988

    Google Scholar 

  45. Webb B II, ed.: Fundamentals of Dairy Chemistry, 2nd Edition. Westport, CT, The Ari Publishing Co, Inc., 1973

    Google Scholar 

  46. Harris S, McClenaghan M, Simons JP, Ali S, and Clark AJ: Gene expression in the mammary gland. J. Reprod. Fert 88: 707–715, 1990

    Article  CAS  Google Scholar 

  47. Clark AJ, Ali S, Archibald AL, Brown P, Harris S, McClenaghan M, Prowse C, Simons JP, Whitelaw CBA, and Wilmut I: The molecular manipulation of milk composition. Genome 31: 950–955, 1989

    Article  PubMed  CAS  Google Scholar 

  48. Reimerdes EH: Natural protease activity in milk. J Dairy Sci 66: 1591, 1983

    Article  PubMed  CAS  Google Scholar 

  49. Korycka-Dahl M, Ribadeau-Dumas B, Chene N, Martal J: Plasmin activity in milk. J Dairy Sci 66: 704, 1983

    Article  CAS  Google Scholar 

  50. Ebert KM, Papaioannou VE: In vivo culture of embryos in the mature mouse oviduct. Theriogenology 31: 299, 1989

    Article  PubMed  CAS  Google Scholar 

  51. Wall RJ, Hawk HW: Development of centrifuged cow zygotes cultured in rabbit oviducts. J Reprod Fert 82: 673, 1988

    Article  CAS  Google Scholar 

  52. Wall RJ, Pursel VG, Hammer RE, Brinster RL: Development of porcine ova that were centrifuged to permit visualization of pronuclei and nuclei. Biol Reprod 32: 645, 1985

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. American Red Cross, Rockville, MD, 20855, USA

    Rekha Paleyanda, Janet Young & William Drohan

  2. Virginia Polytechnic Institute, Blacksburg, VA, 24061, USA

    William Velander

Authors
  1. Rekha Paleyanda
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  2. Janet Young
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  3. William Velander
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  4. William Drohan
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Editor information

Editors and Affiliations

  1. Jerome H. Holland Laboratory, American Red Cross Blood Services, Rockville, Maryland, USA

    Leon W. Hoyer  & William N. Drohan  & 

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© 1991 Springer Science+Business Media New York

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Paleyanda, R., Young, J., Velander, W., Drohan, W. (1991). The Expression of Therapeutic Proteins in Transgenic Animals. In: Hoyer, L.W., Drohan, W.N. (eds) Recombinant Technology in Hemostasis and Thrombosis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3698-7_13

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  • DOI: https://doi.org/10.1007/978-1-4615-3698-7_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6644-7

  • Online ISBN: 978-1-4615-3698-7

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