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Studies on Expression and Regulation of 5-Lipoxygenase in Human B Lymphocytes

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Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury

Part of the book series: Developments in Oncology ((DION,volume 71))

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Abstract

The expression of the 5-lipoxygenase and the 5-lipoxygenase activating protein (FLAP) genes in human tonsillar B cells and in a lymphoblastoid B cell line was demonstrated at the transcriptional level by the polymerase chain reaction (PCR) technique. Intact B cells produced very low amounts of leukotriene (LT) B4 and 5-hydroxyeicosatetraenoic acid upon stimulation with the calcium ionophore A23187 and arachidonic acid in comparison to those formed by sonicates of these cells. Incubation of intact lymphoblastoid B cells with the glutathione depleting agent, azodicarboxylic acid bis[dimethylamidel] (diamide), prior to the addition of the calcium ionophore A23187 and arachidonic acid, led to the formation of similar amounts of LTB4 as those produced by sonicated cells. These results indicate that the glutathione status is of importance for the activity of 5-lipoxygenase in B lymphocytes.

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References

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Author information

Authors and Affiliations

  1. Department of Physiological Chemistry, Karolinska Institutet, S-10401, Stockholm, Sweden

    P.-J Jakobsson, B. Odlander, D. Steinhilber, H.-E Claesson & B. Samuelsson

  2. Department of Medical Cell Genetics, Karolinska Institutet, S-10401, Stockholm, Sweden

    A. Rosen

Authors
  1. P.-J Jakobsson
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  2. B. Odlander
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  3. D. Steinhilber
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  4. A. Rosen
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  5. H.-E Claesson
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  6. B. Samuelsson
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Editor information

Editors and Affiliations

  1. Department of Gynecological Endocrinology, Klinikum Steglitz, Free University Berlin, Berlin, Germany

    Santosh Nigam

  2. Department of Radiation Oncology, Wayne State University, Detroit, MI, USA

    Kenneth V. Honn

  3. Department of Biochemistry, Vanderbilt University, Nashville, TN, USA

    Lawrence J. Marnett

  4. Department of Radiation Biochemistry, Armed Forces Radiobiology Research Institute, Bethesda, MD, USA

    Thomas L. Walden Jr.

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© 1993 Springer Science+Business Media New York

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Jakobsson, PJ., Odlander, B., Steinhilber, D., Rosen, A., Claesson, HE., Samuelsson, B. (1993). Studies on Expression and Regulation of 5-Lipoxygenase in Human B Lymphocytes. In: Nigam, S., Honn, K.V., Marnett, L.J., Walden, T.L. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury. Developments in Oncology, vol 71. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3520-1_7

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  • DOI: https://doi.org/10.1007/978-1-4615-3520-1_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6562-4

  • Online ISBN: 978-1-4615-3520-1

  • eBook Packages: Springer Book Archive

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