Abstract
5-lipoxygenase (5LO) catalyzes oxygenation of arachidonic acid at C-5 leading to 5(S)hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid (5-HPETE), and the further conversion of 5- HPETE to leukotriene A4 (LTA4) (1). Complementary DNAs for human and rat 5LO have been cloned (2,3,4). The cDNA sequences have also been determined for four other mammalian lipoxygenases and for four plant lipoxygenases. Upon comparison of the ten different lipoxygenase amino acid sequences, six conserved histidine residues have been recognized. These histidine residues have been implicated as possible metal ligands in lipoxygenases, and may thus be important for enzyme activity. In this study we have determined the effects of mutagenesis of these histidines, on the enzyme activity of recombinant 5LO expressed in Escherichia coli.
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Zhang, Y., Rådmark, O., Samuelsson, B. (1993). Mutagenesis of Six Conserved Histidines in Human 5-Lipoxygenase, Effects on Enzyme Activity. In: Nigam, S., Honn, K.V., Marnett, L.J., Walden, T.L. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury. Developments in Oncology, vol 71. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3520-1_1
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DOI: https://doi.org/10.1007/978-1-4615-3520-1_1
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