Abstract
In the chronically diabetic heart, it has been reported that the activity of several enzymes bound to cell structures, such as sarcoplasmic reticular Ca++ ATPase, sarcolemmal Na+-K+ ATPase and myosin ATPase is depressed and that the isoenzyme distribution of myosin ATPase is altered (1,2). However, the changes in the activity and isoenzyme distribution of creatine kinase (CK: EC 2.7.3.2) and lactate dehydrogenase (LD: EC 1.1.1.27) which are mainly “soluble” enzymes and clinically important markers of myocardial necrosis (3) have not been reported in the diabetic heart previously. Therefore, in this study we measured total and isoenzyme activities of myocardial CK and LD in rats with insulin dependent diabetes and compared them with control. Since intracellular calcium overload has been suggested in the pathogenesis of diabetic cardiomyopathy (1,2,4), we also measured the activity of the same (iso)enzymes in the myocardium of rats chronically injected with isoproterenol, a pure ß-adrenergic stimulant, which develop cardiomyopathy with intracellular calcium overload (5).
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© 1992 Springer Science+Business Media New York
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Hashimoto, H. et al. (1992). Myocardial Isoenzyme Distribution in Chronic Diabetes: Comparison with Isoproterenol-Induced Chronic Myocardial Damage. In: Nagano, M., Mochizuki, S., Dhalla, N.S. (eds) Cardiovascular Disease in Diabetes. Developments in Cardiovascular Medicine, vol 130. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3512-6_21
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DOI: https://doi.org/10.1007/978-1-4615-3512-6_21
Publisher Name: Springer, Boston, MA
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