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Changes in Cell Morphology, [Ca2+]i and pHi During Metabolic Inhibition in Isolated Myocytes of Diabetic Rats Using Dual-Loading of Fura-2 and BCECF

  • Chapter
Cardiovascular Disease in Diabetes

Part of the book series: Developments in Cardiovascular Medicine ((DICM,volume 130))

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Abstract

Diabetes Mellitus (DM) has been shown to be associated with heart failure in the absence of atherosclerosis (1,2), suggesting a diabetic cardiomyopathy (3,4). The mechanism of heart failure due to the diabetic cardiomyopathy remains to be elucidated. The small vessel disease (5) and the abnormalities of subcellular mechanisms such as myosin ATPase (6) and myosin isoenzymes (7), have been reported in DM myocardium. Recently, abnormalities of Ca2+ metabolism have been reported in DM myocardium, which showed decreased Ca2+-ATPase of sarcoplasmic reticulum (SR) (8,9) and sarcolemma (10). It has also been reported that the activity of Na+/Ca2+-exchange was lower in DM myocardium (11). Previous reports have suggested the possibility of the Ca2+ overload in diabetic cardiomyopathy (11).

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Author information

Authors and Affiliations

  1. Third Department of Internal Medicine, Hamamatsu University School of Medicine, Hamamatsu, Japan

    H. Hayashi, N. Noda, H. Miyata, S. Suzuki, A. Kobayashi & N. Yamazaki

  2. Hamamatsu Photonics K.K., Hamamatsu, Japan

    M. Hirano & T. Kawai

  3. Medical Photonics, Hamamatsu University School of Medicine, Hamamatsu, Japan

    T. Hayashi

Authors
  1. H. Hayashi
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  2. N. Noda
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  3. H. Miyata
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  4. S. Suzuki
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  5. A. Kobayashi
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  6. M. Hirano
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  7. T. Kawai
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  8. T. Hayashi
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  9. N. Yamazaki
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano  & Seibu Mochizuki  & 

  2. Division of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla

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© 1992 Springer Science+Business Media New York

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Hayashi, H. et al. (1992). Changes in Cell Morphology, [Ca2+]i and pHi During Metabolic Inhibition in Isolated Myocytes of Diabetic Rats Using Dual-Loading of Fura-2 and BCECF. In: Nagano, M., Mochizuki, S., Dhalla, N.S. (eds) Cardiovascular Disease in Diabetes. Developments in Cardiovascular Medicine, vol 130. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3512-6_18

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  • DOI: https://doi.org/10.1007/978-1-4615-3512-6_18

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6558-7

  • Online ISBN: 978-1-4615-3512-6

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