Genetically Engineered Bacteria to Identify and Produce Anti-Viral Agents

  • Robert H. Grafstrom
  • Katherine Zachariasewycz
  • Richard A. Brigandi
  • Timothy M. Block
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 312)


We have prepared a strain of Escherichia coli that expresses both the HIV protease and a Tet protein which has been modified to contain the HIV protease recognition sequence. When the protease is expressed, the bacteria will not grow in the presence of tetracycline. However, when the protease is inhibited the bacteria can grow in tetracycline containing media (Block and Grafstrom 1990). We have selected spontaneously arising Tet resistant mutants and have screened them for those that could be producing an inhibitor of HIV protease. The problems in the construction of this strain and the characterization of the various Teti mutants are discussed.


Human Immunodeficiency Virus Antiviral Agent Tetracycline Resistance Tetracycline Resistance Gene Mutant Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media New York 1992

Authors and Affiliations

  • Robert H. Grafstrom
    • 1
  • Katherine Zachariasewycz
    • 1
  • Richard A. Brigandi
    • 1
  • Timothy M. Block
    • 1
  1. 1.Department of Microbiology and Immunology Jefferson Medical CollegeThomas Jefferson UniversityPhiladelphiaUSA

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