Diagnostic Virology — Then and Now
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Several technical approaches are required for the laboratory diagnosis of viral infections. For example, over a 30-year span, laboratory methods have ranged from serology for demonstrating acute phase infection or for measuring immunity or evidence of past infection with a virus and use of cell cultures for demonstration of cytopathic effects (CPE) to amplification and detection of nucleic acid sequences of agents by the polymerase chain reaction (PCR). Between these extremes, enzyme immunoassay (EIA), immunologic detection of early antigens, and nucleic acid probes have expanded our ability to diagnose viral infections much more rapidly than in the past. Because the types and prevalence of viral infections vary according to the specific clinical practice, i,e, children’s hospital, tertiary care clinic, or State Public Health Laboratory, each individual virology laboratory must design their service tests to support these special needs. In a children’s hospital, methods for the diagnosis of respiratory tract are of greatest significance, whereas detection of herpesvirus infections is most important in tertiary care practice. Further, epidemiologic goals of State Health Laboratories may indicate use of serology for enterovirus serotyping or conventional tube cell cultures exclusively for detection of influenza virus CPE.
KeywordsRespiratory Syncytial Virus Diagn Microbiol Infect Nucleic Acid Probe Shell Vial Enterovirus Serotyping
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- Aurelius, E., Johanson, B., Sköldenberg, Staland, A. and Forsgren, M. 1991, Rapid diagnosis of herpes simplex encephelitis by nested polymerase chain assay of cerebrospinal fluid,Lancet, 1:89.Google Scholar
- Baker, D. A., Gonik, B., Milch, P.O., Berkowitz, A. Lipson, S., and Verma, U., 1989, Clinical evaluation of a new herpes simplex virus ELISA: a rapid diagnositc test for herpes simplex virus,Obste Gynecol., 73:322.Google Scholar
- Fedorko, D.P., Ilstrup, D.M., and Smith, T.F., 1988, Effect of age of shell vial monolayers on detection of cytomegalovirus from urine specimens, J Clin Microbiol., 27:2107.Google Scholar
- Jiwa, N.M., Van Gemert, G.W., Raap, A.K. Van de Rijke, F.M., Mulder, A., Lens, P.F., Salimana, M.M., Zwaan, F.E., Van Dorp, W., and Van der Ploeg, M., 1989, Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction,Transplantation, 48:72.CrossRefPubMedGoogle Scholar
- Minnich, L.L., Smith, T.F. Ray, C.G., and Specter, S., 1988, Rapid detection of viruses by immunofluorescence, Cumitech, 24:1.Google Scholar
- Paya, C.V., Holley, K.E., Wisner, R.H., Balasubramanian, K., Smith, T.F., Espy, M.J., Ludwig, J., Batts, K.P., Hermans, P.E., and Krom, R.A.F. 1990, Early diagnosis of cytomegalovirus hepatitis in liver transplant recipients: role of immunostaining, DNA hybridization and culture of hepatic tissue,Hepatology, 12:119.CrossRefGoogle Scholar
- Swierkosz, E.M., Flanders, R., Melvin, L., Miller, J.D., and Kline, M.W., 1989, Evaluation of the Abbott TESTPACK RSV enzyme immunoassay for detection of respiratory syncytial virus in nasopharngeal swab specimens, J Clin Microbiol., 27:199.Google Scholar
- Tac, P., Aarnes, S.L., de la Maza, L.M., and Peterson, E.M., 1989, Detection of herpes simplex virus by 8 h in shell vial cultures with primary rabbit kidney cell,J Clin Microbiol., 27:199.Google Scholar