Summary
We have targeted mRNA sequences encompassing the translation initiation codon of the essential herpes simplex virus type 1 (HSV-1) IE3 gene with three kinds of anti-sense molecule. Addition of a 15mer oligodeoxyribonucleoside methylphosphonate to tissue culture cells resulted in suppression of viral replication. HSV-1 replication was also inhibited in cultured cells containing anti-sense vectors expressing transcripts complementary to the IE3 mRNA. We have also constructed a ribozyme which upon base pairing with the target 1E3 mRNA induces cleavage at the predicted GUC site. A major obstacle to anti-sense studies in animals is drug delivery of preformed anti-sense molecules to ganglionic neurons, the site of HSV latency and reactivation. We speculate as to how this may be accomplished through carrier compounds which are taken up by nerve terminals and transported by retrograde axoplasmic flow. By the same route, HSV itself may be used as an anti-sense vector.
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Cantin, E.M., Podsakoff, G., Willey, D.E., Openshaw, H. (1992). Antiviral Effects of Herpes Simplex Virus Specific Anti-Sense Nucleic Acids. In: Block, T.M., Jungkind, D., Crowell, R.L., Denison, M., Walsh, L.R. (eds) Innovations in Antiviral Development and the Detection of Virus Infections. Advances in Experimental Medicine and Biology, vol 312. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3462-4_14
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DOI: https://doi.org/10.1007/978-1-4615-3462-4_14
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