Abstract
This work deals with detailed investigations of the dynamics of the dielectric relaxations in hydrated lysozyme powders and in plant tissue in the temperature region where transitions have been reported to occur. We use the method of thermally stimulated depolarization currents (TSDC), which is very sensitive to transitions and has been widely used in the study of glass transitions in synthetic polymeric systems. The relaxations are due to proton transport, side chains reorientation and space charge polarization. Two features of the relaxations, namely the dependence of their dynamics on water content and the dependence of their activation energies on temperature, reveal, in analogy to synthetic polymeric systems, the existence of two glass transitions, probably due to the hydration water and the matrix structure.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
W. Doster, A. Bachleitner, R. Dunau, M. Hiebl, and E. Luscher, Thermal properties of water in myoglobin crystals and solutions at subzero temperatures, Biophys.J. 50: 213 (1986).
F. Parak and H. Hartmann, Structural disorder in myoglobin at low temperatures, in “Tunneling”, J. Jortner and B. Pullman, eds., Reidel, Dordrecht (1986).
R.J. Williams and A.C. Leopold, The glassy state in corn embryos, Plant Physiol. 89: 977 (1989).
W. Doster, S. Cusack, and W. Petry, Dynamic instability-of liquidlike motions in a globular protein observed by inelastic neutron scattering, Phys.Rev.Lett. 65: 1080 (1990).
F. Parak, M. Fischer, and G.U. Nienhaus, The similarity in the dynamics of myoglobin and glycevol as seen from Mossbauer spectroscopy on 57Fe, J.Mol.Liq. 42: 145 (1989).
H. Frauenfelder, F. Parak and R.D. Young, Conformational substates in proteins, Ann.Rev.Biophys.Biophys.Chem. 17: 451 (1988).
I.V. Kurinov, Yu.F. Krupyanskii, A.R. Pachenko, I.P. Suzdalev, I.V. Uporou, K.V. Shaitan, A.B. Rubin, and V.I. Goldanskii, Intramolecular dynamics of hydrated DNA studied by Royleigh scattering of Mossbauer radiation, Hyp.Int. 58: 2355 (1990).
E.R. Andrew, D.J. Bryant, and T.Z. Rizvi, The role of water in the dynamics of proton relaxation of proteins, Chem.Phys.Lett. 95: 463 (1983).
G. Carreri, G. Consolini and F. Bruni, Proton tunneling in hydrated biological tissues near 200 K, Biophys.Chem. 37: 165 (1990).
I.E.T. Iben, D. Braunstein, W. Doster, H Frauenfelder, M.K. Hong, J.B. Johnson, S. Luck, P. Ormos, A. Schulte, P.J. Steinbach, A.H. Xie, and R.D. Young, Glassy behaviour of a protein, Phys.Rev.Lett. 62: 1916 (1989).
J.van Turnhout, Thermally stimulated discharge of electrets, in “Topics in Applied Physics, Vol. 33, Electrets”, G.M. Sessler, ed., Springer, Berlin (1980).
L.S.A. Smith and V. Schmitz, The effect of water on the glass transition temperature of poly (methylmethacrylate),Polymer 29: 1871(1988).
B.B. Sauer, P. Avakian, H.W. Starkweather, Jr., and B.S. Hsiao, Thermally stimulated current and dielectric studies of poly (aryl ether ketone ketone), Macromolecules 23: 5119 (1990)
J.P. Crine, A new analysis of the results of thermally stimulated measurements in polymers, J.Appl.Phys. 66: 1308 (1989).
P. Pissis and A. Anagnostopoulou-Konsta, Protonic percolation on hydrated lysozyme powders studied by the method of thermally stimulated depolarization currents, J.Phys.D 23: 932 (1990).
P. Pissis, The dielectric relaxation of water in plant tissue, J. Exp. Botany 41: 677 (1990).
P. Pissis, D. Diamanti and G. Boudouris, Depolarization thermocurrents in frozen aqueous solutions of glucose, J.Phys.D 16: 1311 (1983).
P. Pissis, Dielectric studies of protein hydration, J. Mol. Liq. 41: 271 (1989).
R. Pethig, Dielectric studies of proton transport in proteins, Ferroelectrics 86: 31 (1988).
P. Pissis, A. Anagnostopoulou-Konsta, and L. Apekis, A dielectric study of the state of water in plant stems, J. Exp.Botany 38: 1528 (1987).
P. Pissis, A. Anagnostopoulou-Konsta, L. Apekis, D. Daoukaki-Diamanti and C. Christodoulides, Dielectric effects of water in water-containing systems, J. Non-Crystalline Solids 131–133: 1174 (1991).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 Springer Science+Business Media New York
About this chapter
Cite this chapter
Pissis, P. (1992). Glass Transitions in Biological Systems. In: Bountis, T. (eds) Proton Transfer in Hydrogen-Bonded Systems. NATO ASI Series, vol 291. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3444-0_17
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3444-0_17
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6524-2
Online ISBN: 978-1-4615-3444-0
eBook Packages: Springer Book Archive