Abstract
Molecular interactions driven by GTP binding and its subsequent hydrolysis have emerged in such diverse areas as protein synthesis, polymerisation/depolymerisation of cytoskeletal proteins and transduction of signals which are perceived initially by cell surface located receptors (Bourne, Sanders, and McCormick, 1990). Within the latter category of interactions, which are effected by the signal transducing G-proteins two super-families are evident. These are the monomeric or small G-proteins, typified by Ras and related proteins (Barbacid, 1987; Burgoyne, 1989) and the heterotrimeric G-proteins (Bimbaumer, Abramowitz, and Brown, 1990; Kaziro, Itoh, Kozasa, Nakafuku, and Satoh, 1991). Whilst members of the former family have undoubtedly been identified in plant cells (Drobak, Allan, Comerford, Roberts, and Dawson, 1988) this report will mainly concern itself with evidence for, and progress in, the isolation of members of the heterotrimeric G-protein family.
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Abbreviations
- G-protein:
-
GTP-bindingprotein
- GTPγS:
-
guanosine-5’ -0-(3-thiotriphosphate)
- MEGA-9:
-
nanoyl-N-methylglucamide
- CHAPS:
-
3-[(cholamidopropyl)-dimethyllammonio]-1-propane-sulphonate
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© 1992 Springer Science+Business Media New York
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White, I.R., Wise, A., Finan, P.M., Clarkson, J., Millner, P.A. (1992). GTP-Binding Proteins in Higher Plant Cells. In: Cooke, D.T., Clarkson, D.T. (eds) Transport and Receptor Proteins of Plant Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3442-6_17
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DOI: https://doi.org/10.1007/978-1-4615-3442-6_17
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